摘要
采用纸片扩散法测定金黄色葡萄球菌(以下简称金葡菌)对6种抗生素的敏感性。结果表明,在测定的121株金葡菌中,耐苯唑西林金葡菌(MRSA)为26株,占21.5%;对苯唑西林敏感菌(MSSA)为93株,占76.9%;中介菌株为2株,占1.6%。金葡菌对6种抗生素耐药率最低的是头孢噻肟为2株,占1.6%。mecA基因的PCR扩增结果显示:所有的MSSA mecA基因均阴性,中介株mecA基因阳性1株,而MRSA中mecA基因阳性株为9株。MSSA对大部分抗生素仍保持良好的敏感性,而MRSA表现为多重耐药性,PCR技术可以作为快速检测金葡菌耐药基因的有效方法。
The susceptibility of 121 strains of Staphylococcus aureus isolated from bovine mastitis to six kinds of antibiotics was determined by disk diffusion method. The results showed that 26 strains occupied 21.5% were methicillin - resistant Staphylococcus aureus （ MRSA）, while 93 strains occupied 76. 9% were susceptible to oxacillin （ M$SA）, and 2 strains were intermediary. There were only 2 strains of Staphylococcus aureus had the lowest resistant rate to cefotaxime, which occupied 1.6%. The PCR results showed that the mecA gene of all oxacillin - sensitive strains were negative, while that of one intermediary strain and 9 MRSA strains was positive. The MSSA strains still maintained good susceptibility to most of antibiotics, but the MR- SA strains showed multi -drug resistance. PCR technology can be used as a rapid and effective way to detectthe drug resistant genes of Staphylococcus aureus.
出处
《山东农业科学》
2012年第3期13-16,共4页
Shandong Agricultural Sciences
基金
山东省中青年科学家基金(BS2009NY002)
现代农业(奶牛)产业技术体系科学家岗位
山东省科技攻关课题(2009GG20002032)
关键词
金黄色葡萄球菌
耐药性
耐药基因
Staphylococcus aureus
Resistance
Drug resistant gene
