摘要
目的比较耐甲氧西林金黄色葡萄球菌(M RSA)和甲氧西林敏感的金黄色葡萄球菌(M SSA)对氨基糖苷类药物耐药性和耐药基因携带的不同。方法运用K-B法和PCR方法分别对16株M RSA和24株M SSA 4种氨基糖苷药物(丁胺卡那霉素、奈替米星、庆大霉素、妥布霉素)耐药性和氨基糖苷类耐药基因进行检测。结果M RSA与M SSA对氨基糖苷类药物耐药率分别为93.8%,62.5%,100%,100%和0.0%,0.0%,29.2%,33.3%,三种氨基糖苷类药物耐药基因aac(6')/aph(2'')、aph(3')III、an t(4',4'')携带率分别为68.8%,62.5%,6.3%和29.2%,8.3%,0.0%。结论M RSA较M SSA对氨基糖苷类药物有着更高的耐药性和耐药基因携带率。M RSA较M SSA对氨基糖苷类药物存在着更为复杂的耐药机制。
Objective Study the differences of aminoglycoside-resistance and the presence of resistant gene between methicillin-resistant Staphylococcus aureus （MRSA） and methicillin-Susceptible Staphylococcus aureus（MSSA）. Methods 16 MRSA and 24 MSSA strains were used in this study. The aminoglycoside resistant genes were detected by PCR and aminoglycoside resistance was tested by Kirby-Bauer method to 4 kind of antibiotics （Amikacin Gentamicin Netilmicin Tobramycin）. Result The aminoglycoside resistance rates to 4 kind of aminoglycoside antibiotics of MRSA and MSSA were 93.8%, 62. 5%, 100%, 100% and 0. 0%, 0. 0%, 29. 2% and 33.3% respectively,positive rates by PCR for aac （6′）/aph （2″）,aph（3′）Ⅲ,ant（4′,4″） were 68.8%,62.5%,6.3%,29.2%,8.3 and 0.0% respectively. Conclusion Compare to MSSA,MRSA presents high resistant rate and resistant gene positive rate to aminoglycoside. MRSA contains more complex aminoglycoside resistant mechanism.
出处
《现代检验医学杂志》
CAS
2006年第5期36-38,共3页
Journal of Modern Laboratory Medicine
