摘要
目的应用PCR扩增法检测耐甲氧西林葡萄球菌(MRS)的mecA基因。方法临床分离161株葡萄球菌,采用PCR扩增法鉴定MRS的mecA基因,与Oxacillin纸片扩散法和mecA基因法检测比较MRS株菌的差异。结果 mecA基因法鉴定阴性而Oxacillin纸片扩散法鉴定为耐药株,3株纸片扩散临界耐药,mecA基因法鉴定阳性,两种方法的符合率为96.89%。结论 PCR扩增法mecA基因检测技术可以准确、快速鉴定纸片扩散法无法确定的临界耐药株。
Objective To detect mecA gene of methicillin-resistant staphylococcus by PCR amplification method. Methods A total of 161 clinical staphylococcal isolates were collected to identify mecA gene in methicillin-resistant Staphylococcus（ MRS） by PCR. Then Oxiacillin disk diffusion method and mecA gene PCR method were compared to find the difference of MRS strains. Results Four Staphylococcus strains were found negative by PCR amplification of mecA gene,but identified as MRS by Oxacillin disk diffusion method. Three Staphylococcus strains were found critical resistant by Oxacillin disk diffusion method, but identified as MRS by PCR amplification of mecA gene. The coincidence rate of the two methods was 96. 89%. Conclusion PCR amplification method of mecA gene is superior to disk diffusion method in identification of MRS strains,especially for borderline-resistant strains.
出处
《中国卫生检验杂志》
北大核心
2014年第8期1143-1143,1150,共2页
Chinese Journal of Health Laboratory Technology
