摘要
【目的】研究影响牡丹试管苗多酚氧化酶(PPO)酶学特性的因素,为牡丹的高效组培提供参考。【方法】以牡丹品种"乌龙捧盛"为供试材料,采用分光光度计比色法,研究温度、pH值、PPO提取液添加量及底物的类型和浓度对牡丹PPO活性的影响。【结果】以邻苯二酚为底物时,牡丹PPO的最适反应温度与最适pH分别为25℃和6.0,最适PPO提取液添加量为1mL。牡丹PPO的最适作用底物为咖啡酸,其最适浓度为0.08mol/L。【结论】通过选择合适的pH值和培养温度,同时结合化学抑制剂调控牡丹PPO活性,可以达到在牡丹组织培养中减少褐化现象、提高试管苗生根率的目的。
[Objective] The research aimed to study the influence factors of the polyphenol oxidase characteristics of peony plantlet in vitro. [Method] With cultivar of ＂Wulongpengsheng＂ as experimental materials,the effects of temperature,pH value,PPO content,substrate type and concentration on PPO ac- tivity of Paeonia suffruticosa Andr were studied using spectrophotometry method. [Result] With catechol as substrate, the optimum reaction temperature and pH respectively were 25 ℃ and 6.0, the optimal adding amount of PPO 1 mL,the optimum substrate of PPO coffee acid,and the optimum concentration of coffee acid 0.08 mol/L. [Conclusion] Browning phenomenon can be induced and the rate of tissue culture plant- lets can be improved by selecting the appropriate pH,temperature,and combining with the chemical inhibi- tors to regulate and control peony PPO enzymatic activity.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2012年第3期154-157,162,共5页
Journal of Northwest A&F University(Natural Science Edition)
基金
国家自然科学基金项目(31071816)
关键词
牡丹
多酚氧化酶
酶学特性
peony plantlet
polyphenol oxidase
enzymology characteristics
