摘要
A20/AN1锌指蛋白普遍存在于真核生物中,在胁迫响应引起的信号传导过程中发挥着重要作用。本文利用生物信息学和RT-PCR方法从低温干旱胁迫的沙冬青叶片组织中分离锌指蛋白基因AmSTZF,该基因编码一条含172个氨基酸残基的多肽,含A20和AN1两个锌指蛋白保守结构域,属A20/AN1锌指蛋白。生物信息学分析显示该蛋白定位于细胞质中,具有磷酸化位点,参与细胞转录调控。RT-PCR分析表明:低温和干旱胁迫处理的沙冬青叶片中,AmSTZF的表达增强,初步推测该基因与沙冬青低温、干旱胁迫响应相关。
A20/AN1 zinc finger protein widely exists in eukaryote and plays a central role in signal transduction pathway involved in stress response. We cloned zinc finger gene AmSTZF from cold- and drought-treated Ammopiptanthus mongolicus leaves by bio-informatics and RT-PCR. The cDNA of AmSTZF encodes a polypeptide with 172 amino acids,which contains A20 and AN1 conserved domains and belongs to A20/ AN1 zinc finger protein. Bio-informatics predicts that AmSTZF has phosphorylation sites, exists in cytoplasm and is involved in the regulation of gene transcription. RT-PCR analysis shows that the expression of AmSTZF is up-regulated under cold- and drought-stresses and that AmSTZF is related to the response to drought and chilling stresses.
出处
《北京林业大学学报》
CAS
CSCD
北大核心
2012年第2期103-108,共6页
Journal of Beijing Forestry University
基金
国家自然科学基金项目(30700559
30671476)
关键词
沙冬青
A20/AN1锌指蛋白
序列分析
表达分析
Ammopiptanthu mongolicus
A20/AN1 zinc finger protein
sequence analysis
expressionanalysis
