摘要
为研究狗枣猕猴桃锌指蛋白(AkCHY)功能提供参考,利用生物信息学工具和方法,对其蛋白质的理化性质、亲水/疏水性、跨膜结构、结构功能域、二级结构、三级结构、蛋白质修饰位点及同源性等进行分析和预测。结果表明:AkCHY的cDNA全长为1768bp,编码区(CDS)为837bp,是由279个氨基酸构成的亲水性非稳定蛋白,属于ZF-CHY和zinc-ribbon-6蛋白家族成员(ID:11342810),主要构件为α-螺旋和不规则卷曲,包含锌指结构特有的RING型结构域;与其他15种植物的CHY氨基酸序列进行对比,狗枣猕猴桃与中华猕猴桃原变种的亲缘关系较近。
In order to provide reference for application of function of zinc finger protein(AkCHY)from Actinidia kolomikta,the physicochemical properties,hydrophilic/hydrophobic,transmembrane structure,structural functional domain,secondary structure,tertiary structure,protein modification site and homology of zinc finger protein were analyzed and predicted by using bioinformatics tools and methods.The results showed that the length of AkCHY cDNA was 1 768 bp and the coding area was 837 bp coding 279 amino acids.AkCHY is a member of zf-chy and zinc-ribbon-6 protein families(ID:11342810).The main components are alpha-helix and irregular coil,which contain the RING type domain characteristic of zinc finger structure.Compared with the CHY amino acid sequences of 15 other plants,A.kolomikta is closely related to A.chinensis var.chinensis.
作者
陈鑫
李然红
刘丹
王立凤
刘思言
CHEN Xin;LI Ranhong;LIU Dan;WANG Lifeng;LIU Siyan(Mudanjiang Normal University,Mudanjiang,Heilongjiang 157012,China)
出处
《贵州农业科学》
CAS
2019年第9期106-109,共4页
Guizhou Agricultural Sciences
基金
黑龙江省教育厅项目（1354MSYYB006、1354MSYYB007、1354ZD005）
黑龙江省教育厅备案项目（1351MSYYB007）
黑龙江药用植物开发与利用研究（1353PT007）
黑龙江省大学生创新创业训练计划项目（201810233053）
