摘要
Objective： To investigate the effect of puerarin on intedeukin （IL）-8 mRNA expression and the protein release in the co-culture of human bronchial epithelial （BEAS-2B） cells and human neutrophils. Methods： BEAS- 2B cells and neutrophills were cultured separately and co-cultured with puerarin （50, 100, and 200 ug/mL） for a predetermined time. Cytokines in culture supematant were evaluated by protein array and IL-8 quantified by enzymelinked immunosorbent assay （ELISA）. IL-8 mRNA expression was evaluated by real-time quantitative polymerase chain reaction （real-time qPCR）. Results： The co-culture of BEAS-2B cells and neutrophils exhibited synergistic effects on IL-8 mRNA expression in BEAS-2B cells, but not in neutrophils after 12 h incubation （P〈0.01）, as compared with that in BEAS-2B cells or neutrophils alone. IL-8 protein release in the culture supernatant was obviously elevated when BEAS-2B cells were co-cultured with human neutrophils as compared with that in the supematant of BEAS-2B cells or neutrophils alone after incubated for 2, 6, 12, and 18 h （P〈0.01）. Treatment with puerarin could significantly down-regulate the expression of IL-8 mRNA in BEAS-2B cells and IL-8 release in the supematant of the co-culture of BEAS-2B cells and neutrophils （P〈0.01）. Conclusion： Puerarin could exhibit anti-inflammatory activity by suppressing IL-8 production from the co-culture of human bronchial epithelial cells and neutrophils.
Objective： To investigate the effect of puerarin on intedeukin （IL）-8 mRNA expression and the protein release in the co-culture of human bronchial epithelial （BEAS-2B） cells and human neutrophils. Methods： BEAS- 2B cells and neutrophills were cultured separately and co-cultured with puerarin （50, 100, and 200 ug/mL） for a predetermined time. Cytokines in culture supematant were evaluated by protein array and IL-8 quantified by enzymelinked immunosorbent assay （ELISA）. IL-8 mRNA expression was evaluated by real-time quantitative polymerase chain reaction （real-time qPCR）. Results： The co-culture of BEAS-2B cells and neutrophils exhibited synergistic effects on IL-8 mRNA expression in BEAS-2B cells, but not in neutrophils after 12 h incubation （P〈0.01）, as compared with that in BEAS-2B cells or neutrophils alone. IL-8 protein release in the culture supernatant was obviously elevated when BEAS-2B cells were co-cultured with human neutrophils as compared with that in the supematant of BEAS-2B cells or neutrophils alone after incubated for 2, 6, 12, and 18 h （P〈0.01）. Treatment with puerarin could significantly down-regulate the expression of IL-8 mRNA in BEAS-2B cells and IL-8 release in the supematant of the co-culture of BEAS-2B cells and neutrophils （P〈0.01）. Conclusion： Puerarin could exhibit anti-inflammatory activity by suppressing IL-8 production from the co-culture of human bronchial epithelial cells and neutrophils.
基金
Supported by the National Natural Science Foundation of China (No.30873419)
