摘要
目的探讨银杏叶提取物(Ginkgo biloba leaf extract,GbE)对细菌脂多糖(LPS)诱导人宫颈癌细胞(HeLa细胞)炎症应激作用的影响。方法将HeLa细胞分成LPS组、GbE+LPS干预组和对照组3组,通过细胞骨架染色了解细胞结构的稳定性;应用OLYMPUS-AU2700自动生化分析仪测定细胞上清液C反应蛋白(CRP)、前白蛋白(PA)和乳酸脱氢酶(LDH)含量,了解细胞应激反应情况和细胞死亡情况。结果 GbE+LPS组培养48h后细胞骨架较松散,稳定性明显低于LPS组和对照组;GbE+LPS干预组培养8 h后细胞上清液CRP含量显著低于LPS组(tGbE+LPS=-3.649,P<0.05),而LDH含量高于LPS组,但差异无统计学意义(tGbE+LPS=-3.56,P>0.05);各组各时间段细胞上清液PA含量差异均无统计学意义(P>0.05)。结论 GbE具有介导HeLa炎症细胞凋亡及减轻细胞炎症反应的作用。
Objective To explore the effects of Ginkgo biloba extract（GbE） on lipopolysaccharides（LPS）-induced inflammation of HeLa cells.Methods HeLa cells were assigned into three groups： LPS group,GbE＋LPS group and control group.Stability of cellular structure was observed with cytoskeleton dyeing.CRP,PA and LDH in the cell supernatant fluid were assessed by OLYMPUS-AU2700 automatic biochemistry analyzer.Results The cytoskeleton of cells in GbE＋LPS group was incompact at 48 h,with cellular structure stability significantly lower than that in LPS group or control group.CRP in cell supernatant fluid in GbE＋LPS group was significantly lower than that in LPS group（tGbE＋LPS=-3.649,P0.05）.Although LDH in cell supernatant fluid in GbE＋LPS group was higher than that in LPS group,significant difference was revealed（tGbE＋LPS=-3.56,P0.05）.No significant difference in cell supernatant PA was revealed among the three groups（P0.05）.Conclusion GbE promotes apoptosis of HeLa cells and alleviates the LPS-induced inflammation.
出处
《广东医学》
CAS
CSCD
北大核心
2012年第4期442-444,共3页
Guangdong Medical Journal
基金
广东省中医药局建设中医药强省立项资助课题(编号:2010213)
广州市中医药科研资助项目(编号:2010A06
2010A08)
