摘要
目的 :建立小鼠舌体移植瘤颈淋巴结转移模型 ,探讨癌周淋巴管的病理改变与淋巴道转移的关系。方法 :将U14的腹水瘤细胞移植于昆明小鼠舌体内 ,分期分批切取舌体组织 ,改良 5′ 核苷酸酶组化染色法显示癌周淋巴管。结果 :小鼠舌体接种U14肿瘤细胞后颈淋巴结转移率可达 70 % ;U14肿瘤细胞进入淋巴管腔的方式主要有 2种 ,即通过正常管壁开放间隙和造成管壁缺损进入淋巴管腔。结论 :随着移植瘤的生长 ,瘤周淋巴管不断扩张 ,管壁结构出现不完整 ,有利于肿瘤细胞进入淋巴管腔进而发生淋巴结转移。
Objective:①To establish a model of neck lymphatic metastasis of cancer by injecting tumor cells into the rat tongue; ②To observe the relationship between pathologic changes of perineoplastic lymphatic in different stages of implanted tumor growth and lymphatic metastasis of tumor cells. Methods: 60 Kunming rats with injection of 5×10\+6 U14 tumor cells into each rat tongue were chosen and randomized into 6 groups. In every 5 days, specimens of rat tongues with implanted tumor and neck lymph nodes with possible metastatic tumor cells were removed from each group of rats. Cryosections of rat tongue were processed with a modified enzyme histochemical staining technique, using 5′ nucleotidase with HE to demonstrate lymphatic capillaries. Attention was paid to pathologic change of lymphatic capillaries in perineoplastic area of rat tongue in different growth stages of implanted tumor. Routing wax sections of lymph nodes processed with HE staining were examined under light microscopy to determine whether there were any metastasis tumor cells. Results: ①Metastasis to neck lymph nodes could be observed 15 days later after injection of U14 tumor cells into rat tongue. The situation of metastasis to lymph nodes varied greatly from tumor cells in bundle within marginal sinus to complete occupation of lymph nodes by U14 cells. ②Tumor cells penetrated the lymphatic capillary by passing singly or in clumps between endothelial cells or through some destroyed endothelia. A great number of lymphatic with dilated cavity was found nearby the tumor tissue which facilitated tumor cells to enter the lymphatic cavity. ③There were much more amount of lymphatic capillaries with dilated cavity around tumor mass comparing with that of normal tongue of the controll group rats, and the number of lymphatic capillaries with opening cavity tend to increase with growth of implanted tumor. Conclusion: Direct and objective view of lymphatic capillaries can be obtained by means of the modified enzyme histochemical staining technique
出处
《华西口腔医学杂志》
CSCD
北大核心
2000年第1期5-8,共4页
West China Journal of Stomatology
基金
国家自然科学基金!(编号 95 70 767)
