摘要
目的:探讨检测非小细胞肺癌(NSCLC)患者外周血Lunx mRNA表达对于诊断肿瘤微转移的临床价值。方法:采用荧光定量RT-PCR技术分别对98例NSCLC患者和20例正常对照组外周血Lunx mRNA的表达进行检测分析。结果:NSCLC组患者外周血Lunx mRNA的阳性率为74.5%,显著高于对照组的15%(x^2=25.642,P<0.05);Ⅲ期NSCLC患者的阳性率显著高于Ⅰ期和Ⅱ期患者的阳性率(x^2=21.014,P<0.05);淋巴结转移阳性和阴性患者的外周血中CK19mRNA阳性率分别为93.2%和46.2%,差异有统计学意义(x^2=27.372,P<0.05);不同细胞分化程度与NSCLC患者外周血微转移阳性率有密切关系(x^2=30.004,P<0.05);NSCLC患者外周血微转移与年龄、性别、吸烟史、原发肿瘤部位无密切关系(P>0.05)。结论:Lunx mRNA的高表达可能与非小细胞肺癌的微转移有关,可作为检测NSCLC患者微转移的分子标志物,其有助于早期诊断肺癌转移,指导临床分期和治疗。
Objective： To evaluate the clinical significance of Lunx mRNA detection in the peripheral blood of patients with non-small cell lung cancer （ NSCLC ）. Methods： Lunx mRNA expression was detected by fluorescence quantitative reverse transcrip- tion polymerase chain reaction in the peripheral blood from 98 NSCLC patients and a control group of 20 healthy adults. Results： The positive rate of Lunx mRNA in peripheral blood in the NSCLC group was 74.5%, significantly higher than that in the control group （ Z = 25.642, P 〈 0.05 ）. The expression rate increased significantly as the clinical stage progressed （ ~2 = 21.014, P 〈 0.05）. Significant difference was found among the different lymph node metastasis status （ ;~2 = 27.372, P 〈 0.05 ） and different cell differentiation status （ Z2 = 30.004, P 〈 0.05）. No significant difference was noted between the positive rates of Lunx mRNA for different ages, gender, history of smoking, and primary tumor position of NSCLC （ P 〉 0.05 ）. Conclusion： Higher Lunx mRNA expression in the peripheral blood of NSCLC patients may be related to the micro-metastasis of the disease and might be a valuable marker for micrometastasis. Lunx mRNA detection is significant for the early prediction of NSCLC prognosis and metastasis.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2012年第2期74-76,84,共4页
Chinese Journal of Clinical Oncology
基金
广东省医学科学技术研究基金(编号:A2009217)资助~~
