摘要
目的:探讨蛇床子素、补骨脂素及丹皮酚对人乳腺癌高转移MDA-MB-231BO细胞株的体外抑制及侵袭作用的影响,并初步探讨其作用机制。方法:采用均匀设计实验方案进行组方设计;MDA-MB-231BO细胞体外侵袭实验,实时荧光聚合酶链反应(RT-PCR)检测,观察药物处理后细胞的侵袭情况及对人转化生长因子TGF-β1基因的表达情况。结果:获取蛇床子素、补骨脂素、丹皮酚3个药物的最佳配伍比例为10.00:7.78:6.67;单体组和阳性药物组在体外作用24h后,可以显著抑制MDA-MB-231BO细胞的侵袭(P<0.01)。结论:中药单体组蛇床子素、补骨脂素、丹皮酚对乳腺癌MDA-MB-231BO细胞的生长具有抑制作用,有效抑制其侵袭和转移,其机制可能与抑制TGF-β1基因的表达有关。
Objective： To study the effects of Osthole,Psoralen,Paeonol on cell lines in vitro on proliferation and invasion of breast cancer MDA-MB-231BO and to study the anti-cancer mechanism of the Chinese Herb active components.Methods： The best combination dose of osthole,psoralen,paeonol on inhibition breast cancer MDA-MB-231BO cell lines in vitro was obtained after adopting the uniform experiment design;MDA-MB-231BO in vitro invasion assay was observed in cell invasion after drug treatment,and Real-time Quantitative RT-PCR experiment was observed the gene expression after drug treatment.Results： The best matched compatibility of Osthol,psoralen,Paeonol was 10.00：7.78：6.67;MDA-MB-231BO cells invasion was significantly inhibited after 24 hours drug treatment by The Chinese monomer groups and Zoledronic acid for injection group;TGF-β1 gene expression after drug treatment was significantly reduced compared with the control group（P0.01）.Conclusion： The best matched compatibility of osthol,psoralen,paeonol can inhibit the growth of MDA-MB-231BO cells and can effectually inhibit the invasion of MDA-MB-231BO cells in vitro.The mechanism may be related to down-regulating the expression of TGF-β1.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2012年第2期430-433,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金资助项目(No.30772811)
上海市教委曙光跟踪计划基金资助项目(No.08GG12)~~
