摘要
该研究构建了由黄瓜素基因5′端310bp启动子序列驱动β-葡萄糖醛酸酶(GUS)报告基因的植物表达载体pPZP-CGN,通过花粉管通道法将植物表达载体pPZP-CGN导入甜瓜,并采用荧光法定量测定转基因植株中GUS活性。结果显示,gus基因在果实中高表达,而在根、茎、叶等组织中表达活性很低,表明黄瓜素基因上游310bp启动子具有指导外源基因在果实中高效特异表达的特性。
In order to study the function of cucumisin promoter in Cucumis melo L.cv.Hetao,a plant expression vector pPZP-CGN was constructed containing the cucumisin 310 bp promoter region,the coding sequence of β-glucuronidase（GUS） as a reporter gene and nos terminator.The melon cultivar Hetao was transformed with pPZP-CGN by pollen tube pathway transformation method.Fluorescence analysis of GUS activity in transgenic melon plants revealed that the promoter was able to direct gus gene expression at high levels in fruits,but almost not in leaves,stems and roots.It was determined that the 310 bp promoter region of cucumisin gene is responsible for high level fruit-specific expression.
出处
《西北植物学报》
CAS
CSCD
北大核心
2011年第11期2153-2157,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家基础科学人才培养基金(J0730648)
国家自然科学基金(30660111)
国家转基因植物研究与产业化专项(JY04-B-02)
