摘要
通过PCR扩增,从拟南芥中克隆出ats1A基因启动子(包括叶绿体转运肽),将此启动子与GUS基因相连构建植物瞬时表达载体,用基因枪法将之导入烟草进行瞬时表达。GUS基因检测分析表明,ats1A基因启动子能特异的启动GUS基因在烟草叶片中高效表达。
The 1 117 bp 5' flanking sequence of Arabidopsis thaliana ats1A gene including the promoter region and the coding region of chloroplast transition peptide was cloned by PCR .The fragment and the β-glucuronidase gene were fused to construct p18T1a- GUS expression vector and the expression vector was transformed to tobacco(NC89) by bombardment. Histochemically analysis 24h after bombardment show that GUS expressed in leaves and stems of tobacco.
出处
《西北植物学报》
CAS
CSCD
2004年第10期1856-1860,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
陕西省农业分子生物学重点实验室基金项目
关键词
拟南芥
atslA基因启动子
叶绿体转运肽
烟草
微弹轰击
Arabidopsis thaliana
ats1A gene promoter
tobacco
chloroplast transit-peptide
a particle bombardment
