摘要
在水稻基因组芯片分析的基础上,克隆到一个在水稻中高水平表达基因OsSG1 5’末端启动子区域1.6-kb的DNA片断,即Ospz1启动子,构建了由Ospz1启动子引导的GUS重组基因,并经农杆菌介导将重组基因导入到水稻中。对转基因水稻植株中GUS活性的定性测定结果表明,Ospz1启动子可驱动GUS报告基因在转基因水稻植株叶片、芽和根中高效表达,而在其它器官中不表达或表达活性极弱,表现出组织特异性。Ospz1启动子可用于农作物生物技术的遗传改良。
To clone a rice gene promoter,a highly active gene,OsSG1,was selected from rice based on the data obtained by microarray analysis.A 1.6-kb DNA fragment,Ospz1,at the 5＇ promoter region of the gene was cloned and sequenced.In order to investigate the promoter activity in transgenic rice,Ospz1 was ligated to the GUS gene in vector pCAMBIA1301,and the constructed vector was delivered into rice by Agrobacterium-mediated method.GUS histochemical staining assay showed that Ospz1 directed the GUS gene expression in leaves,sprouts and roots of transgenic rice plants,but not in other organs.Ospz1 is valuable and may be used as a promoter in the genetic improvement of crops through biotechnology.
出处
《农业现代化研究》
CSCD
北大核心
2011年第2期234-237,共4页
Research of Agricultural Modernization
基金
中国科学院“百人计划”项目(编号:02200420062903)
