摘要
目的建立准确、灵敏的液相色谱-串联质谱方法,检查中药制剂中非法添加的10种糖皮质激素。方法采用液相色谱-串联质谱分析法,以SHIMADZU Shim-pack VP-ODS C18(4.6 mm×150 mm,5μm)为色谱柱;流动相:乙腈-10 mmol/L乙酸铵溶液(30∶70);流速:0.3 ml/min;柱温:30℃;质谱条件:以正离子模式进行质谱数据采集。结果 10种糖皮质激素高效液相色谱图谱分离度良好,质谱分辨率符合要求。结论此方法简便、快速,灵敏度高,专属性强,可作为检测中药制剂中非法添加10种糖皮质激素的有效手段。
Objective To establish an high performance liquid chromatography-mass/mass spectrometry method for the identification of ten glucocorticoids which may be illegally adulterated in Traditional Chinese Medicine preparations. Methods The determination was carried out by liquid chromatography-mass/mass spectrometry in the positive mode using a VP-ODS C18（4.6 mm ×150 mm,5μm） column and acetonitrile/10 mmol/L ammonium acetate （30： 70） as the eluting solvent. The flow rate was 0.3 ml/min and the column temperature was 30℃. Resuits A good resolution of the ten kinds of glucocorticoids was reached in the high performance liquid chromatography-mass analysis. Conclusion The method is simple, rapid, sensitive and specific and can be used to detect the ten glucocorticoids illegally added in in Traditional Chinese Medicine preparations.
出处
《解放军药学学报》
CAS
2011年第6期524-526,共3页
Pharmaceutical Journal of Chinese People's Liberation Army
关键词
液相色谱-串联质谱分析法
糖皮质激素
中药制剂
high performance liquid chromatography-mass/mass
glucocorticoids
Traditional Chinese Medicine preparations
