摘要
以温度敏感型甘蓝型油菜雄性不育系160S为试验材料,并以可育油菜31C为对照,对160S育性转换过程中叶片和花药保护性酶的活性等进行了研究,研究中测定了160S在育性转换温度敏感时期超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)等抗氧化酶活性以及丙二醛(MDA)含量。结果显示,160S在育性转换期保护性酶活性降低,MDA过量积累;而作为对照的可育油菜31C保护性酶活性较高,MDA含量相对较低;以上差异和变化主要出现在花药中,表明不育系160S发生育性转换的一个重要因素是其花药中抗氧化酶活性系统产生了变化,使得活性氧自由基清除能力严重下降,从而进入活性氧自由基和MDA相互促进过量积累的不良循环。研究提示过量积累的活性氧自由基和MDA可能破坏了花药中细胞的膜结构,导致花药中花粉正常发育过程受阻而不能形成有活力的花粉母细胞,最终导致不育现象的发生。
This research studied the activities of the protective enzyme in the leaves and anthers during the fertility transformation of the thermo-sensitive male-sterile line 160S of Brassica napus and selected the fertile rape as the contrast. The protective enzyme activities of superoxide dismutase （SOD）, peroxidase （POD）, catalase （CAT）, ascorbate-peroxidase （APX） and malondialdehyde （MDA） content were measured. The results showed that the protective enzyme activities of 160S decreased and MDA content accumulated excessively during the fertility conversion, but the fertile line of 31C was of higher protective enzyme activity at the same temperature of the same period, while MDA content was relatively low and the anthers did not atrophy. Those differences of protective enzyme activity and MDA content accumulation were mainly occurred in the anthers of male sterile line 160S under natural temperature conditions. The resuits showed that one important factor of the fertility transformation of 160S probably was that anti-oxidation enzyme activities were abnormal which result in that the eliminating ability of reactive oxygen species decreased, and the reactive oxygen species and MDA accumulated excessively which probably destroyed the cell membrane. As a result, the pollens developed abnormally, then could not develop the pollen mother cell and behaved abortion traits eventually.
出处
《重庆师范大学学报（自然科学版）》
CAS
北大核心
2012年第1期82-86,共5页
Journal of Chongqing Normal University:Natural Science
基金
重庆市高等教育教学改革研究项目(No.103116)
关键词
温敏雄性不育
甘蓝型油菜
抗氧化酶
丙二醛
thermo-sensitive male sterility
Brassica napus
anti-oxidation enzyme
MDA
