摘要
[目的]研究顶果木离体培养最佳方法。[方法]以顶果木种子获得的无菌苗进行离体培养,通过诱导丛生芽的发生,获得再生植株。[结果]采用MS+6-BA 0.8～3.0 mg/L+NAA 0.2～0.5 mg/L培养基、改良MS+6-BA 0.2～1.2 mg/L+NAA 0.18～0.2 mg/L培养基均能保持无菌苗的生长,其中改良MS+6-BA 0.2 mg/L+NAA 0.18 mg/L培养基最适宜无菌苗生长,但培养40 d后,未见芽苗分化;将无菌苗转入改良MS+6-BA 0.5～1.2 mg/L+NAA 0.2 mg/L培养基中培养,均能诱导芽的分化和增殖,培养20 d后,芽繁殖系数可达1.72～2.30,其中改良MS+6-BA 1.2 mg/L+NAA 0.2 mg/L培养基芽繁殖系数达2.30,总体芽苗生长情况差异不明显。[结论]改良MS+6-BA 1.2 mg/L+NAA 0.2 mg/L培养基为诱导顶果木最适培养基。
[Objective] The aim of the study was to seek the best method on tissue culture of Acrocarpus fraxinifoliu.[Method] The multi-buds induction and regeneration system of Acrocarpus fraxinifolius from seed in vitro was preliminary investigated.[Result] The results showed that the MS＋6-BA 0.8-3.0 mg/L＋NAA 0.2-0.5 mg/L culture medium,modified MS＋6-BA 0.2-1.2 mg/L＋NAA 0.18-0.2 mg/L culture medium could maintain seedling growth,which modified MS＋6-BA 0.2 mg/L＋NAA 0.18 mg/L culture medium was the most suitable for seedling growth,but the bud differentiation were not happened after 40 days.The modified MS＋6-BA 0.5-1.2 mg/L＋NAA 0.2 mg/L culture medium could induce bud differentiation and proliferation.The propagation coefficient of the bud could reach 1.72-2.30 after 20 days,which the propagation coefficient was 2.30 that culture in modified MS＋6-BA 1.2 mg/L＋NAA 0.2 mg/L medium,the overall shoots growth were not significant.[Conclusion] The modified MS＋6-BA 1.2 mg/L＋NAA 0.2 mg/L culture medium was the most suitable for tissue culture of Acrocarpus fraxinifoliu.
出处
《安徽农业科学》
CAS
2012年第3期1457-1458,共2页
Journal of Anhui Agricultural Sciences
关键词
顶果木
无菌苗
离体培养
Acrocarpus fraxinifolius
Aseptic seedlings
In vitro culture
