摘要
将鸭甲肝病毒鸡胚化弱毒MY株Vp1基因原核表达工程菌(pET32a+Vp1,BL21(DE3)PlysS)对基因Vp1进行大量表达和纯化,得到鸭甲肝弱毒MY株Vp1重组蛋白作为抗原制备亚单位疫苗,免疫雏鸭,用间接ELISA检测方法对免疫后抗体生成水平进行监测,并通过动物攻毒试验评价免疫保护效果。结果显示:Vp1重组蛋白免疫家兔后血清检测琼扩效价≥1∶64,细胞中和试验效价为1/45;雏鸭免疫后抗体生成水平呈明显上升趋势,对攻毒起到了保护作用。首次揭示了DHAV-1结构蛋白Vp1刺激机体产生抗体使雏鸭获得保护的能力。
Prokaryotic expression vector（pET32a＋Vp1,BL21（DE3）PlysS） was employed to express the Vp1 gene of duck hepatitis virus MY strain.The purified recombinant protein was used as antigen to produce subunit vaccine.And then ducklings were inoculated with the subunit vaccine,and the indirect ELISA was used to determine the level of antibody.Meanwhile,challenge test was performed to evaluate the effect of immunoprotection.The results showed that after rabbits were immunized by the Vp1,the agar gel precipitation titer in serum was more than 1：64,and neutralization titer was 1/45 After ducklings were immunized,increasing tendency of antibody was observed,which could prevent ducklings from duck hepatitis virus challenge.This study revealed the ability of Vp1 expressed in organism and prevent ducklings from duck hepatitis virus for the first time.
出处
《中国兽医杂志》
CAS
北大核心
2012年第1期6-9,共4页
Chinese Journal of Veterinary Medicine
基金
四川省科技厅应用基础研究"鸭肝炎鸡胚化弱毒MY株的生物学特性及其疫苗生产条件的研究"(2008JY0069)
关键词
鸭肝炎
鸡胚化弱毒
Vp1重组蛋白
免疫保护
duck hepatitis
chicken-embryo-adapted attenuated strain
recombinant Vp1 protein
immunoprotection
