摘要
目的研究齐墩果酸(OA)对H_2O_2诱导血管平滑肌细胞(VSMCs)凋亡的影响。方法采用贴块法培养大鼠主动脉VSMCs,随机分为对照组、H_2O_2组、H_2O_2+OA组、阻断剂组。采用Hoechst 33342染色和Annexin V/FITC染色检测各组细胞凋亡情况;Western blot检测各组细胞中磷酸化Akt蛋白表达变化。结果与对照组比较,H_2O_2组细胞凋亡率明显升高,p-Akt蛋白表达明显降低(P<0.05);与H_2O_2组比较,H_2O_2+OA组细胞凋亡率明显降低,p-Akt蛋白表达明显升高(P<0.05);与H_2O_2+OA组比较,阻断剂组细胞凋亡率明显升高,p-Akt蛋白表达明显降低(P<0.05)。荧光显微镜显示,对照组细胞核呈蓝色,H_2O_2组细胞核呈致密浓染,H_2O_2+OA组细胞核呈蓝染,有少量细胞核致密浓染,阻断剂组细胞核呈致密浓染。结论 OA能减轻H_2O_2导致的VSMCs凋亡,其机制可能与激活细胞内磷脂酰肌醇-3激酶/Akt信号通路引起下游促生存基因的表达有关。
Objective To investigate the protective effect of oleanolic acid（OA） against vascular smooth muscle cells（VSMCs） apoptosis induced by hydrogen peroxide（H202） and to explore the related mechanism. Methods Rat aortic VSMCs were isolated,cultured and divided into normal control group, H2O2 group, H2O2＋ OA group and H2O2 ＋ OA ＋ wortmannin group. Results Compared with normal control group, H2O2 significantly induced VSMCs apoptosis and reduced the protein level of p-Akt（P〈0.05）. Compared with H2O2 group, OA attenuated H2 O2-induced apoptosis of VSMCs and up-regulated the protein level of p-Akt（P〈0.05）. Compared with H2O2-V OA group,pretreatment of VSMCs with wortmannin could increase the apoptosis of VSMCs and reduce the protein level of p-Akt（P〈0.05）. Conclusions OA can reduce the apoptosis of VSMCs induced by H2O2 ,which may be brought about through PI3K/Akt signaling pathway.
出处
《中华老年心脑血管病杂志》
CAS
北大核心
2012年第1期66-68,共3页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
基金
国家自然科学基金(30470452)
关键词
齐墩果酸
过氧化氢
肌
平滑
血管
细胞凋亡
基因表达
氧化性应激
oleanolic acid
hydrogen peroxide
muscle, smooth, vascular
apoptosis
gene expression
oxidative stress
