摘要
目的探讨SOD在扶正活血清热方治疗大鼠急性放射性肠炎(ARE)中的作用机理。方法将48只成年健康Wistar雄性大鼠随机分为6组(8只/组),即空白对照组(BC)、生理盐水组(PS;9 g/L)、地塞米松组(DS;1.425mg/kg)、扶正活血清热方高、中、低剂量组(HF、MF和LF;16.67g/kg、12.5g/kg和8.33g/kg)。各组大鼠于造模后第2天开始用生理盐水或相应药物灌肠干预5d(5毫升/只),7d后麻醉采血测定血清中SOD活性,取放射区域直肠组织进行组织学观察。结果组织学变化证实DS、HF和MF组均可有效治疗模型大鼠ARE;DS和MF组血清中SOD活性水平略低于BC组(P>0.05),但明显高于PS和LF组(F=14.199,P<0.001)。结论有效维持体内SOD活性的正常水平可能是扶正活血清热方治疗ARE作用机制中的重要环节之一。
Objective To evaluate the possible role of superoxide dismutase （SOD） in the mechanism of Fuzhenghuoxueqingrefang＇s treating acute radiation enteritis （ ARE ） in rats. Methods Forty eight Wistar male rats were randomly divided into 6 groups（8 rats each group）, which consisted of blank control（BC）, physiological saline group （ PS; 9 g/L ） , dexamethasone group （ DS; 1.425mg/kg ） , and high ,medium and low Fuzhenghuoxueqingrefang groups （HF, MF, and LF; 16.67g/kg, 12.5g/kg, and 8.33g/kg）. The physiological saline and the drugs were administrated separately at the first day after abdominal irradiation by daily rectal clysma for 5 days. The morphologic changes （HE stain） and the serum activity of SOD were detected at the seventh day after abdominal irradiation separately. Results No obvious morphologic changes were observed in the DS, HF, and MF groups with histopathologic evaluation（HE stain）, and the activity of serum SOD in the DS and MF groups were obviously higher than the PS and LF groups（F= 14.199, P〈0.001）,but lower than the BC group（P〉0.05）. Conclusion Sustaining the routine activity of serum SOD effectively might play an important role in the mechanism of Fuzhenghuoxueqingrefang＇s treating ARE in rats.
出处
《医学研究与教育》
CAS
2011年第6期9-12,共4页
Medical Research and Education
基金
河北省2010年科技支撑计划项目(102761101)
河北省中医药管理局科学技术研究课题(2008071)
河北大学大学生科技创新项目(2011099)
河北大学博士基金(2011213)
