摘要
目的:探讨肠复康方对急性放射性肠炎大鼠一般情况及小肠黏膜组织形态学的影响。方法:以X线直线加速器给予实验大鼠全腹照射(DT9.0 Gy),建立急性放射性肠炎大鼠模型。实验大鼠随机分成正常对照组、模型对照组、肠复康方低、中、高剂量组。造模成功后连续灌胃30 d,观察对比大鼠精神状态、摄食进水、排便情况及体质量变化,处死后取相应部位的小肠用HE染色法在光学显微镜下观察肠黏膜上皮的组织形态学改变。结果:造模成功后肠复康方低、中、高剂量组大鼠精神状态、饮食进水、排便情况均优于模型对照组;肠复康方中剂量组大鼠体质量高于模型对照组,差异有统计学意义(P<0.01)。肠复康方低、中、高剂量组大鼠小肠黏膜组织形态学改变程度低于模型对照组,肠复康方中剂量组黏膜组织形态学改变程度与模型对照组、肠复康方低剂量组比较,差异均有统计学意义(P<0.05);肠复康方低剂量组黏膜组织形态学改变程度与高剂量组比较,差异无统计学意义(P>0.05)。结论:肠复康方可改善急性放射性肠炎大鼠一般情况,具有修复小肠黏膜组织形态学改变的作用。
Objective： To explore the effects of Changfukang decoction （肠复康方） on general condition and histomorphology on small intestinal mucosa in rats with acute radiation enteritis. Methods： An abdominal irradiation in a dose of 9.0 Gy X-ray of linear accelerator was performed once on a group of rats to establish a model of acute radiation enteritis. The experimental rats were randomly divided into five groups： normal control group, model control group, Changfukang decoction low dose group, middle dose group and high dose group. After the model was established, all rats were given corresponding drugs by garage once a day for 30 days. In the meantime, their mental state, dietary intake, defecation and body mass changes were observed and compared. After the rats were sacrificed, corresponding intestinal tissues were observed, the change of histomorphology on the intestinal mucosal epithelial cells under optical microscope was determined by H-E staining. Resuits： After the model was established, medication groups mental state, dietary intake and defecation were better than model control group＇s. Compared with model control group, medication groups had significant increase in body mass changes, typically Changfukang decoction middle dose group （P〈0.01）. Compared with model control group, medication groups had a better way to relieve the change of histomorphology on small intestinal macosa. The difference between treatment with middle dose of Cbangfukang decoction compared with model control group, low dose group was statistically significant （P〈0.05）, but between low and high dose group it has no significance （P〉0.05）. Conclusion： Chang-fu-kang decoction had an effect on improving general condition and healing the change of histomorphology on small intestinal mucosa in rats with acute radiation enteritis.
出处
《中医药导报》
2016年第12期17-20,共4页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
湖南省中医药管理局一般课题(201177)
关键词
肠复康方
放射性肠炎
大鼠
小肠黏膜
组织形态学
Changfukang Decoction
radiation enteritis
rats
small intestinal mucosa
histomorphology
