摘要
以福建道地瓜蒌带腋芽的茎段为外植体,MS为基础培养基,研究不同激素水平对福建道地瓜蒌组织培养的影响,探讨福建道地瓜蒌快速繁殖的有效途径。结果表明:瓜蒌带腋芽的茎段接种于MS+1.0mg.L-16-BA蔗糖培养基上,15d后外植体出现许多绿色的小芽点,芽点健壮;30d后,诱导出的丛生芽数平均达6.5个,长势快,平均出芽率达81.25%。分离正常的幼芽在MS+0.4mg.L-1 NAA的生根培养基上,效果最好,15d后长出不定根,再生成完整的瓜蒌植株,平均生根5条,根系粗壮、发达,生根整齐,生根率达100%。通过瓜蒌带腋芽茎段的组织培养,可以对瓜蒌种苗进行脱毒和品种更新复壮,解决瓜蒌以种子繁育雌雄比例不协调的问题,可在短期内大量繁殖瓜蒌雌雄种苗,具有成本低,效益高的特点。
Effect of hormone application on tissue culture of Trichosanthes kirilowii Maxim was investigated.Stems with axillary buds were used as explantlet,and MS for the basic medium.A vailable methods for the rapid propagation to clone T.kirilowii Maxim are reviewed.In the experiment,the young adventitious buds continuously appeared for 15 days in the culture medium of MS ＋ 1.0 mg·L-1 BA.In 30 days,the average number of shoots was 6.5,and the average shoot ratio was 81.25%.The excised plantlets could produce root 15 days after inoculation on the MS ＋ 0.4 mg NAA L-1 medium.They continued to gradually develop into normal plants.A single plant had an average of 5 roots.The average rooting percentage was 100%.This rapid propagation method for T.kirilowii Maxim appeared to offer many benefits at low cost.
出处
《福建农业学报》
CAS
2011年第5期774-777,共4页
Fujian Journal of Agricultural Sciences
基金
福建省农业科学院科技创新团队建设基金项目(STIF-Y01)
福建省农业科学院"双百行动"计划项目(sbmb1104-1)
福州市科技计划项目(2011-S-76)
关键词
福建
瓜蒌
组织培养
激素水平
Fujian
Trichosanthes kirilowii Maxim
tissue culture
hormone application
