摘要
采用考马斯亮蓝G-250染料染色法对市售乳品中蛋白质进行测定,在595 nm波长处测定溶液的吸光度。实验结果表明,标准蛋白质在10～80μg/mL之间呈现良好的线性关系。该法简便快速、灵敏度高、重现性好,是测定乳品中蛋白质的有效方法。
We employed the dying method of Coomassie brilliant blue G-250 to determine the protein content of milk, measuring the absorbency at the wavelength of 595 nm. Experiment shows that a good linear relationship exists between the absorbency and the content of standard proteins in the range of 10 - 80 μg/mL. This approach has such advantages as simplicity, rapidity, good reproducibility and sensitivity, especially suitable for the determination of milk protein.
出处
《山东科学》
CAS
2011年第6期53-55,共3页
Shandong Science
关键词
乳品
蛋白质含量
考马斯亮蓝法
测定
milk
content of proteins
Coomassie brilliant blue dying method
determination
