摘要
目的探讨藁本内酯(Z-ligustilide,LIG)对β淀粉样蛋白(Aβ)25-35引起的人脑神经瘤母细胞SH-SY5Y细胞毒性的保护作用。方法 0.1、1.0、2.5、5.0μg/mL LIG作用于SH-SY5Y细胞,24h后以50μmol/LAβ25-35诱导SH-SY5Y细胞凋亡建立阿尔茨海默病体外模型,MTT法检测细胞活性,Western blot法检测细胞中凋亡蛋白含量。结果经Aβ25-35处理后,细胞存活率下降,细胞内凋亡相关蛋白——Bax、cleaved caspase3、细胞色素C、caspase8表达上调,但Bcl-2蛋白表达下降(P均<0.05)。细胞经过LIG一定浓度预处理,细胞存活率提高,凋亡相关蛋白——Bax、cleaved caspase3、细胞色素C、caspase8蛋白表达下降,Bcl-2表达上升(P均<0.05)。结论 LIG具有对Aβ25-35诱导的SH-SY5Y细胞毒性的保护作用,其机制可能是抑制Aβ诱导的细胞凋亡。
Objective To investigate the effects of Z-ligustilide（LIG） on Aβ25-35-induced cytotoxicity in human neuroblastoma SH-SY5Y cells.Methods Cells were incubated with 0.1,1.0,2.5,5.0 μg/mL LIG and then were exposed to 50 μmol/L Aβ25-35 to induce cytotoxicity in SH-SY5Y cell to establish the AD model in vitro.Cell viability was test by MTT method.Proand anti-apoptosis protein levels were investigated by Western blot.Results After exposure to Aβ25-35,the cell viability decreased significantly,and the expression of pro-apoptosis protein——Bax,cleaved caspase 3,cytochrome C,caspase 8 was up regulated while the anti-apoptosis protein——Bcl-2 was down regulated.However,the treatment of LIG（0.1,1.0,2.5,5.0 μg/mL） significantly restored these changes.Conclusion LIG has the protective effect against cytotoxicity induced by in SH-SY5Y cells via inhibit the apoptosis induced by Aβ25-35.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2012年第1期34-37,共4页
Journal of Sichuan University(Medical Sciences)
基金
四川省科技支撑项目(2009SZ0142)资助
