摘要
目的测定川芎挥发油中藁本内酯在大鼠体内的药动学。方法采用RP-HPLC法,以蛇床子素为内标物测定大鼠口服川芎挥发油的β-环糊精包合物后藁本内酯的血药浓度,使用3P97药动学软件计算其药动学参数。结果藁本内酯在大鼠体内符合二室模型,主要的药动学参数:tl/2(α)为(1.429±1.161)h,tl/2(β)为(6.877±2.275)h,t(peak)为(3.401±1.951)h,AUC为(70.87±25.92)μg/mL.h。结论以蛇床子素为内标,RP-HPLC法能够准确、灵敏地测定藁本内酯在大鼠体内的药动学。
Objective To investigate the in vivo pharmacokinetics of ligustilide in Ligusticum chuan- xiong essential oil after ig administration in rats. Methods The concentration of ligustilide in plasma of rats after ig administration of β-cyclodextrin compound in L. chuanxiong enssential oil was determined by RP-HPLC method with the osthole as the internal standard. The plasma concentration-time curve was plotted. The. main pharmacokinetic parameters of ligustilide were obtained by 3P97 software. Results Li- gustilide in rats fits the two compartments model and the main pharmcokinetic parameters were as follows： t1/2（α）, （1.429±1.161） h; t1/2（β）, （6.877±2.275） h; t（peak）, （3.401±1.951） h; and AUC were （70.87±25.92） μg/mL · h, respectively. Conclusion RP-HPLC Method with osthole as the internal standard is accrurate, sensitive, and rapid for the study on pharmacokinetics of ligustiliden in rats in vivo.
出处
《中草药》
CAS
CSCD
北大核心
2009年第2期228-230,共3页
Chinese Traditional and Herbal Drugs
