摘要
研究普鲁兰酶法制备玉米抗性淀粉的工艺。在单因素试验基础上,采用响应曲面法研究pH值、反应温度、反应时间和加酶量对抗性淀粉得率的影响,优化玉米抗性淀粉制备工艺,建立各因素与抗性淀粉得率关系的数学回归模型。确定最佳的制备工艺条件为普鲁兰酶加酶量12.8ASPU/g、反应时间32h、反应温度46.2℃、p H 5.0。在该制备条件下,抗性淀粉得率为4 6.2%。
In this study,resistant starch was prepared from maize starch with pullulanase.Based on one-factor-at-a-time experiments,the main parameters of pullulanase hydrolysis were investigated by response surface methodology（RSM）,including reaction temperature,pH value,reaction time,and pullulanase amount,and a mathematical model describing resistant starch yield was developed.The optimum preparation conditions were achieved as follows： pullulanase amount 12.8 ASPU/g,reaction temperature 46.2 ℃,reaction time 32 h,pH 5.0.Under these conditions,the yield of the maize resistant starch is 46.2%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第22期11-15,共5页
Food Science
基金
江苏省自然科学基金创新学者攀登项目(BK2008003)
江苏畜牧兽医职业技术学院科技项目(YB201004)
江苏省“青蓝工程”资助项目
关键词
抗性淀粉
普鲁兰酶
玉米淀粉
响应面
resistant starch
pullulanase
maize starch
response surface methodology（RSM）
