摘要
目的:建立大鼠跟腱周围炎病理模型,揭示针刺防治肌腱周围炎的作用机制。方法:24只Wistar大鼠随机分为对照组、造模组和针刺干预组,采用"跳跃运动"方法制作大鼠跟腱周围炎的动物模型,6周后取材进行HE染色,免疫组织化学法检测HIF-1α和VEGF的表达。结果:(1)模型组大鼠跟腱及其周围组织出现明显的炎性反应病理特征,腱组织、肌肉以及软骨组织的HIF-1α和VEGF的表达水平均明显高于对照组(P<0.01)。(2)针刺干预组的腱组织和肌肉中HIF-1α和VEGF表达水平高于对照组(P<0.05或P<0.01),软骨组织则均无明显差异,但针刺干预组与模型组比较,腱组织、肌肉以及软骨组织的HIF-1α和VEGF的表达水平明显下降(P<0.05或P<0.01)。结论:"跳跃运动"是建立跟腱周围炎动物模型的可行方法,改善组织内部缺氧环境可能是针刺防治肌腱周围炎重要途径之一。
Objective：To explore the control mechanism of acupuncture revealed paratendinitis,a rat pathological model of paratendinitis was developed.Methods：24 Wistar rats were randomly divided into control group,model group and the acupuncture treatment group.A＂jumping movement＂approach was used to make a rat model of paratendinitis.Six weeks after operation,rat tissue were stained by routine HE.HIF-1α and VEGF expression were detected by Immunohistochemical method.Results：（1）rat tendon and surrounding tissue showed obvious pathological features in Model group.HIF-1α and VEGF expression in Tendon,muscle and cartilage tissue in Model group were significantly higher than in control group（P0.01）.（2）HIF-1α and VEGF expression of tendon and muscle in Acupuncture intervention group were higher than in control group（P0.05 or P0.01）.There was no significant difference in cartilaginous tissue.But HIF-1α and VEGF expression levels of tendon,muscle and cartilage tissue in acupuncture intervention group were significantly decreased,compared with that of in model group（P0.05 or P0.01）.Conclusion：＂Jumping movement＂is a feasible way to built a rat pathological model of paratendinitis.Improving Hypoxic environment within the organization may be one of important ways that acupuncture revealed paratendinitis.
出处
《辽宁中医杂志》
CAS
北大核心
2011年第12期2310-2313,共4页
Liaoning Journal of Traditional Chinese Medicine
基金
江苏省科技厅社会发展项目(BS2006020)
