摘要
生物材料周围的感染和组织损伤是材料表面的黏附细菌引起的。为了阻止感染的发生,将环丙沙星共价结合到聚氨酯骨架中合成了环丙沙星-聚氨酯(CFPU)抗菌材料。在37℃,pH=7.4的PBS缓冲溶液条件下,研究了CFPU的降解情况,并对降解液的抗菌性能及细胞毒性进行了初步研究。研究结果表明,合成的CFPU在模拟正常体液环境下一个月内的药物损失在2%以内,较好地保留了药物。在胆固醇酯酶存在的条件下,药物的释放量明显大于空白实验,并且酶浓度的提高加剧了CFPU中药物的释放,基本达到了控制释放的效果。抗菌实验结果显示,只有酶降解液具有抗菌活性,最低抑菌浓度(MIC)为0.376μg/mL。细胞毒性实验表明降解液呈弱毒性,可以用于体内进行进一步的研究。
The infection and tissue damage around the biomaterials are mainly caused by the bacterial adherence to the surface of the material. A new kind of antibacterial ciprofloxacin- polyurethane （CFPU） was synthesized in order to prevent the infection. The degradation of CFPU in the phosphate-buffer saline （PBS） （pH=7.4, 37℃） was studied. The antibacterial ability and the cytotoxicity of the degradation solutions were assayed. The results showed that ciprofloxacin（CF） was kept effectively in CFPU while the drug loss was below 2% under the mimetic humoral condition. In the presence of cholesteral esterase（CE）, CF release was obviously larger than in the PBS only. Hence, the increase of the concentration of CE accelerated the release of CF, which indicated the control release ability of the material.. The enzymatic degradation showed an ability to kill bacteria and the minimum inhibitory concentration （MIC） was measured as 0. 376μg/mL. The degradation solutions were hypotoxicity according to the MTT assay.
出处
《高分子通报》
CAS
CSCD
北大核心
2011年第12期87-91,共5页
Polymer Bulletin
基金
福建省科技厅资助省属高校项目(2008F50502)
关键词
抗菌
控制释放
聚氨酯
环丙沙星
降解
胆固醇酯酶
Antibacterial； Control release； Polyurethane ；Ciprofloxacin； Degradation； Cholesterol esterase
