摘要
建立了以凝胶渗透色谱(GPC)和高效液相色谱(HPLC)联用技术检测食用油中邻苯二甲酸酯类含量的方法。样品经GPC处理后,采用乙腈/水体系为流动相,ODS柱分离,PDA检测器检测,检测波长224nm。实验结果:该方法在0.1～8.0mg/L范围内线性关系良好;DMP、DEP、BBP、DBP、DNOP的仪器检出限(S/N=3)分别为10、10、25、50、50μg/L,低、中、高三个浓度水平的回收率均在95%～110%之间。结论:该方法准确可靠,精密度较高。
The method for determination of phthalic acid esters in edible oil by gel permeation chromatography and HPLC was described. The chromatographic analysis was achieved after the pretreatment by GPC. The separation was performed in a ODS column,acetonitrile-water as mobile phase. A PDA detector was used to detect the compositions at 224nm. The assay linear calibration range was 0. 1～8. 0mg/L. The limit of quantitation（S/N=3）of DMP,DEP,BBP,DBP,DNOP were 10,10,25,50,50μg/L,respectively. The recoveries at the three spiked levels were ranged from 95% to 110%. The results of repeatability test and reproducibility test provided that this method was repeatable and reproducible.
出处
《食品工业科技》
CAS
CSCD
北大核心
2011年第12期484-485,488,共3页
Science and Technology of Food Industry
基金
四川省公益性科技计划项目(2008NG0008)
