摘要
目的:探讨熊果酸(UA)对糖尿病(DM)大鼠肾组织的保护作用及机制。方法:链脲佐菌素(STZ)诱导糖尿病大鼠模型,随机分为正常对照组(N组)、糖尿病组(D组)、糖尿病+UA治疗组(DM+UA组);EMSA法检测NF-κB活性,RT-PCR和Western blot分别检测P选择素mRNA和蛋白表达。结果:造模成功16周后,和D组相比,D+UA组尿蛋白排泄、肾功能和肾组织病理都得到一定程度改善,且UA治疗明显抑制了DM大鼠肾组织内NF-κB激活和P选择素表达。结论:熊果酸对DM大鼠肾组织具有一定的保护作用,其机制可能通过抑制NF-κB激活和P选择素表达相关。
Objective： To investigate the renal protection of ursolic acid（UA） in diabetic rats and its mechanism.Methods： Diabetic rats were induced by streptozotocin（STZ） and randomly divided into control group（N group）,diabetic group（D group）,and diabetes ＋ UA group（D ＋ UA group）.NF-κB activity was detected by EMSA.The mRNA and protein levels of P-selectin expression were detected by RT-PCR and Western blot.Results： Sixteen weeks after diabetic rat modeling,the urinary protein excretion,renal function and renal pathology of rats in D ＋ UA group had been improved to some extent as compared to D group.And UA treatment was significantly inhibited NF-κB activation and the expression of P selectin in renal tissue of diabetic rats.Conclusion： Ursolic acid has a protective effect,on renal tissue in diabetic rats,which may be associated with the inhibition of NF-κB activation and P selectin expression.
出处
《武汉大学学报（医学版）》
CAS
北大核心
2011年第6期723-726,I0001,共5页
Medical Journal of Wuhan University
基金
湖北省自然科学基金资助项目(编号:2008CDB204)
关键词
糖尿病肾病
熊果酸
核因子-ΚB
P选择素
Diabetic Nephropathy
Ursolic Acid
Nuclear Factor-Κappa B
P-Selectin
