摘要
调查并分离了广东、海南、广西、福建和云南等香蕉枯萎病疫区的一批病原菌样本。利用高效液相色谱—电喷雾离子阱质谱(HPLC-ESI-MS)法对所分离的28个Fusarium oxysporum f.sp.cubense菌株的次生代谢产物进行初步分析。发现了分子量为179D的镰刀菌酸及分子量约为783D的未知产物;利用1H-NMR对一株4号生理小种的未知产物进行了结构测定,结果表明该化合物为白僵菌素。离体试验表明白僵菌素能够导致香蕉假茎腐烂。
The 28 isolates of Fusarium oxysporum f. sp. cubense（Foc）including Race 1 and 4 were collected from the banana Fusarium wilt diseases epidemic areas of Guangdong,Hainan,Guangxi,Fujian and Yunnan provinces. The secondary metabolites were analyzed by HPLC-ESI-MS. We found fusaric acid which the molecular weight is 179 Dalton and another compound with a molecular weight of 783 Dalton. The latter was purified and identified to be beauvericin by 1H-NMR. Experiments in vitro proved that the banana pseudostem rot caused by the beauvericin which was produced by Foc.
出处
《园艺学报》
CAS
CSCD
北大核心
2011年第11期2092-2098,共7页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(30971991)
广东省自然科学基金项目(10151064001000007)
公益性行业(农业)科技专项(200903049)
中非政府间合作项目(2010DFA32470)
关键词
香蕉枯萎病
白僵菌素
结构
毒性
Fusarium wilt of banana
beauvericin
structure
toxicity
