摘要
采用同源克隆及cDNA末端快速扩增法(RACE),从烟草中克隆到1个谷氧还蛋白基因(Grx),命名为NbGRX1。该基因全长1021bp,编码298个氨基酸,具有典型的Grx基因结构域。Real-timeRT-PCR分析表明,该基因能在烟草的根、茎、叶中表达,并且受低温、干旱和高盐诱导。
A cDNA encoding a protein containing the consensus Grx family domain with a CGFS active site was isolated using homologue cloning and RACE methods. Quantitative real-time RT-PCR （qRT-PCR） analysis revealed that NbGRX1 is expressed ubiquitously in tobacco including leaf, root, and stem, and can be induced by low temperature, drought and salt stresses.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2011年第10期47-52,共6页
Journal of Northeast Agricultural University
基金
贵州省科学基金项目(黔科合J字[2011]2399号)
关键词
本氏烟
谷氧还蛋白基因
荧光定量PCR
Nicotiana benthamiana
glutaredoxin gene
quantitative real-time RT-PCR
