摘要
本研究从紫花苜蓿cDNA中克隆到一个MsWRKY11基因,进化分析表明MsWRKY11与蒺藜苜蓿WRKY11亲缘关系最近。MsWRKY11受NaCl、水杨酸(SA)和茉莉酸(JA)的诱导表达。将PHB-MsWRKY11-Flag表达载体转入紫花苜蓿获得超表达紫花苜蓿。250 mmol·L^(-1)NaCl处理下,转基因株系的株高和地上生物量,以及过氧化氢酶(CAT)、过氧化物酶(POD)活性和K^(+)/Na^(+)均明显高于野生型,而Na^(+)含量、电导率、丙二醛(MDA)含量和超氧阴离子含量均显著低于野生型。进一步分析发现盐胁迫下耐盐性相关的关键基因MsNHX1、MsSOS3、MsAPX、MsGRX、MsNAC和MsP5CS的表达量受到强烈诱导,转基因紫花苜蓿叶片中6个基因的表达量高于野生型。上述结果表明,MsWRKY11通过调节细胞K^(+)/Na^(+)内稳态和保护过氧化物酶活性,从而降低Na^(+)对细胞膜结构的破坏,提高紫花苜蓿的耐盐能力。
MsWRKY11 was cloned from the cDNA of Medicago sativa,and transgenic lines of over expressed MsWRKY11(MsWRKY11-OE) in alfalfa were obtained by transplanting PHB-MsWRKY11-Flag expression vector into alfalfa. The effect of MsWRKY11 on alfalfa tolerance to salt stress was studied by treating transgenic lines and wild type(WT)with 0 or 250 mmol·L^(-1) NaCl. The results showed that MsWRKY11 was closely related to Medicago truncatula WRKY11,and the expression of MsWRKY11 was upregulated by addition of NaCl,salicylic acid and jasmonic acid. The growth rates(aboveground biomass and height),peroxidase activity,catalase activity and ratio of K^(+)/Na^(+) were increased in MsWRKY11-OE lines compared with WT,meanwhile the Na^(+) content, relative conductance, malondialdehyde content and superoxide anion content were decreased in MsWRKY11-OE lines under salt stress. The expression of salt-tolerance related genes,including MsNHX1,MsSOS3,MsAPX,MsGRX,MsNAC and MsP5 CS were upregulated under salt stress. Also,the expression of those genes was higher in MsWRKY11-OE lines than in the WT under salt stress. These results indicate that MsWRKY11 was involved in the maintenance of K^(+)/Na^(+) homeostatic status and stimulation of peroxidase activity under salt stress,and through these mechanisms enhanced the salt-tolerance of alfalfa.
作者
王如月
文武武
赵恩华
周鹏
安渊
WANG Ru-yue;WEN Wu-wu;ZHAO En-hua;ZHOU Peng;AN Yuan(School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China)
出处
《草业学报》
CSCD
北大核心
2021年第11期157-169,共13页
Acta Prataculturae Sinica
基金
国家自然科学基金(32071863,31872408)资助。
关键词
紫花苜蓿
WRKY基因
抗盐
转录因子
alfalfa(Medicago sativa)
WRKY
salt-tolerance
transcription factor
