摘要
目的探讨变应性鼻炎患者鼻黏膜中白介素(interleukin,IL)-17A及黏蛋白MUC5AC的表达及相互关系。方法总共纳入14例中-重度持续性变应性鼻炎患者和9例正常对照者,采用定量逆转录聚合酶链反应(qRT-PCR)和免疫组化检测下鼻甲黏膜中IL-17A和MUC5AC的mRNA及蛋白表达情况,评估两者之间的关联性。结果变应性鼻炎患者鼻黏膜中IL-17A和MUC5AC mRNA表达分别比对照组织上升3.7倍和8.9倍,差异有统计学意义(P<0.01),两者的蛋白表达强度也显著高于正常对照组(P<0.05)。而且IL-17A和MUC5AC的mRNA与蛋白表达之间存在显著正相关(r=0.79和r=0.85,P<0.05)。结论 IL-17A可能通过刺激MUC5AC的表达加重变应性鼻炎的临床严重度并且影响其疗效。
Objective To examine the expression of interleukin（IL）-17A and MUCSAC and evaluate the relationship in patients with allergic rhinitis （AR）. Method Fourteen patients with moderate to severe AR and 9 normal controls were enrolled. IL-17A and MUC5AC mRNA in nasal mucosa were determined by quantitative reverse transcription polymerase chain reaction （qRT-PCR）, and the protein was located by immunohistochemistry （IHC）. Results We found that IL-17A and MUCSAC mRNA expression were elevated for 3.9 and 7.9 folds, respectively, with significant difference （P〈0.01）. And IL-17A and MUC5AC protein expression were also found to be increased in AR patients when compared with the normal controls （P〈0.05）. And IL-17A level （mRNA and protein） was found to be positively associated with MUCSAC level （mRNA and protein） （r=0.79 and r=0.85, P〈0.05）. Conclusion The expression of IL-17A is associated with MUC5AC level in patients with AR, which might play important role in enhancing clinical severity of AR and affecting clinical efficacy of intranasal corticosteroid.
出处
《中国中西医结合耳鼻咽喉科杂志》
2011年第5期305-307,313,共4页
Chinese Journal of Otorhinolaryngology in Integrative Medicine
基金
国家自然科学基金面上项目(30872845
81070771)
