摘要
目的建立五味子HPTLC定性鉴别和HPLC含量测定方法。方法以五味子甲素、五味子乙素、五味子醇甲、五味子酯甲为对照品,采用HPTLC法带状点样于GF254板上,以石油醚(30～60℃)-甲酸乙酯-甲酸(15∶5∶1)为展开剂,上行展开8 cm,置于紫外灯254nm下观察,比较不同来源的五味子活性成分的差异。以五味子醇甲为对照品,采用HPLC法,ZORBAX SB-C18(4.6 mm×250 mm,5μm),流动相:甲醇-水(65∶35);流速:1.0 ml.min-1;柱温:室温;检测波长:254 nm,比较不同来源的五味子醇甲的含量。结果 HPTLC法能够准确快速鉴别不同来源的五味子;3种药材中的五味子醇甲含量有差异。结论该方法简便、准确、重现性好,可从定性、定量两方面评价五味子药材质量。
ObjectiveTo establish HPLC and HPTLC methods for identification and determination of Schisandra.MethodsChromatographic conditions were as follows： deoxyschizandrin,γ-Schisandrin,Schisandrin,Schisantherin as control samples;band application on GF254 plate;developer solvent system： ligarine（30～60℃）-ethyl formate-methanoic acid（15∶5∶1）;distance from lower edge of plate 8 mm and observing the fluorescent chromatogram in a UV cabinet at 254 nm.The sample solution was analyzed by HPLC.The assay was performed on a Zorbax SB-C18 column（4.6 mm×250 mm,5 μm）.The mobile phase was： methanol：H2O=（65∶35）,the flow rate was 1.0 ml·min-1,the detection wavelength was 254nm and column temperature was room temperature.ResultsHPTLC method could quickly and accurately identify different sources of Schisandra and three kinds of herbs were different in the content of schisandrin.ConclusionThe methods are simple,accurate and reproducible,which can evaluate the quality of Schisandra qualitatively and quantitatively.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2011年第7期1698-1699,共2页
Lishizhen Medicine and Materia Medica Research
基金
国家"十一五"科技支撑计划重点项目(No.2008BADB4B06)
安徽科技学院项目(No.X201080)
