摘要
对洞庭湖水域野生鲫群体中同时存在的二倍体(2n=100)、三倍体(3n=150±)和四倍体(4n=200)野生鲫血液相关特点进行研究。通过流式细胞仪检测野生鲫倍性,将不同倍性野生鲫进行血细胞涂片观察,使用全自动血液分析仪检测不同倍性野生鲫血红蛋白含量。结果发现,3种不同倍性野生鲫血细胞大小与倍性成正比,且能在三倍体和四倍体野生鲫血细胞中观察到一定数量的由无丝分裂所导致的哑铃型细胞核的存在;同时3种不同倍性野生鲫血红蛋白含量没有明显区别。根据这些结果推测,两种高倍性的野生鲫很可能是通过血细胞的无丝分裂来增加细胞数量,保证其总血红蛋白含量相对稳定,从而进一步保证它们运输氧气的能力。
The blood characteristics of diploid（abbreviated 2nCC,2n=100）,triploid（abbreviated 3nCC,3n=150±）and tetraploid（abbreviated 4nCC,4n=200）crucian carp coexisting in Dongting water system were studied in this research.The ploidy level was examined by flow cytometer.The nuclear traits of erythrocytes of different ploidy crucian carp（abbreviated CC）were observed.The hemoglobin contents of different ploidy CC were measured by blood analyser.The results indicated that the mean nuclear volume of erythrocytes in peripheral blood increases regularly with increasing ploidy.Moreover,some erythrocytes had dumbbell nucleus in the peripheral blood of 3nCC and 4nCC in contrast to 2nCC which resulted from amitosis.No obvious differences in hemoglobin contents were observed among different ploidy CC.Inferred from these results,amitosis that existed in erythrocytes of 3nCC and 4nCC might lead to the similar hemoglobin contents among different ploidy CC,which resulted in no obvious differences in oxygen transporting ability of blood from that of 2nCC.
出处
《水产学报》
CAS
CSCD
北大核心
2011年第10期1488-1493,共6页
Journal of Fisheries of China
基金
国家杰出青年科学基金(30725028)
国家自然科学基金重点项目(30930071)
湖南省自然科学创新研究群体基金项目(10JJ7004)
国家公益性行业科研专项资金(200903046)
教育部博士点基金(20104306110004)
湖南省重点学科建设项目资助
关键词
野生鲫
倍性
血细胞
无丝分裂
crucian carp（Carassius auratus）
different ploidy
erythrocytes
amitosis
