摘要
目的:研究昆明山海棠(THH)对胶原诱导性关节炎(CIA)大鼠模型中低氧诱导因子1α(HIF-1α)表达的影响及其作用机制。方法:建立CIA大鼠模型,随机分组,治疗组分别采用高、中、低剂量THH每天灌胃1次,连续用药30d。动态观察关节炎指数(AI)及关节的病理改变,用RT-PCR及免疫组化染色法分别检测HIF-1αmRNA及其蛋白的表达。结果:THH可明显抑制CIA大鼠的足爪肿胀,明显减轻滑膜增殖及炎症反应,HIF-1α表达明显降低。结论:THH通过降低HIF-1α的表达,减轻CIA模型大鼠关节的炎症反应。
AIM： To investigate the effect of tripterygium hypoglaucum hutch （THH） on the expression of hypoxi induciable factor （HIF-1α） in rats with collagen induced arthritis （CIA） model and its mechanism. METHODS： Establish CIA model rat, group randomly, treat groups with gastric feeding at High, Medium and Low doses of THH once a day, with continuous medication for 30 days. Record arthritis index （AI） and joint pathology change. Detect HIF-1α mRNA and protein expression with PT-PCR and immunohistochemical staining. RESULTS： THH could inhibit paw swelling in CIA rats, reduce synovial proliferation and inflammatory response significantly. CONCLUSION： THH may mitigate joint inflammation of CIA model rat through reduction of HIF-1α expression.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2011年第10期1061-1064,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
广西科学基金资助项目(桂科基0575106)
广西教育厅科研项目(200911MS160)
关键词
昆明山海棠
胶原诱导性关节炎
低氧诱导因子-1Α
大鼠
tripterygium hypoglaucum hutch
collagen induced arthritis
hypoxia induciable factor-1α
rat
