摘要
目的建立高效液相色谱法同时测定复方芩兰口服液中绿原酸和黄芩苷含量的分析方法。方法用自制C8-C13混合型烷基键合硅胶柱(150 mm×4.6 mm,5μm)为固定相,甲醇-0.4%的磷酸水溶液为流动相,在C8-C13柱上梯度洗脱,流速为1mL/min,紫外检测波长为323 nm。结果绿原酸和黄芩苷的线性范围分别为4.1-102.0μg/mL和8.5-212.6μg/mL;相关系数分别为0.999 2和0.999 3;加样回收率分别为97.58%和98.16%;检出限分别为0.02μg/mL,0.05μg/mL;精密度实验RSD分别为1.10%和1.88%;重现性实验RSD分别为1.35%和1.00%;稳定性实验RSD分别为1.34%和1.18%;4批样品中绿原酸的含量在0.331-0.344 mg/mL,黄芩苷的含量在8.778-10.44mg/mL。结论该方法简便,快速,准确,灵敏度高,重现性好,成本低,可用于复方芩兰口服液的质量控制。
Objective To develop an RP-HPLC method for the determination of chlorogenic acid and baicalin in compound Qinlan oral liquid.Methods A C8-C13 mixedalkyl（150 mm×4.6 mm,5 μm）bond silica for reversed-phase HPLC was prepared.The mobile phase was CH3OH-0.4% Phosphoric acid.The flow rate was set at 1.0 mL/min.The detection wavelength was at 323 nm.Chlorogenic acid and baicalin were separated by HPLC with grade elution.Results The linearity ranges of chlorogenic acid and baicalin were 4.1-102.0 μg/mL and 8.5-212.6 μg/mL respectively,the correlations coefficients were 0.999 2 and 0.999 3,the average recoveries of adding sample were 97.58 % and 98.16 %,the detection limits were 0.02 μg/mL and 0.05 μg/mL,the RSD of measurement precisions test were 1.10% and 1.88%,the RSD of reproducibility between tests were 1.35% and 1.00%,the RSD of stability test were 1.34% and 1.18%.The content of chlorogenic acid in compound Qinlan oral liquid was 0.331-0.344 mg/mL,and that of baicalin was 8.778-10.44 mg/mL.Conclusion The method is simple,fast,accurate,sensitive,reproducible and low cost.It is fit for the quality control of compound Qinlan oral liquid.
出处
《湖北中医药大学学报》
2011年第5期30-32,共3页
Journal of Hubei University of Chinese Medicine
