摘要
目的建立测定复方芩兰口服液中绿原酸和黄芩苷的含量分析方法。方法采用高效液相色谱法,色谱柱为C-18烷基硅烷柱,绿原酸：甲醇-0.4%的磷酸水溶液（24∶76）为流动相,检测波长327 nm;黄芩苷：甲醇-水-磷酸（47∶53∶0.2）为流动相,检测波长274 nm;流速1.0 ml/min,柱温30℃。结果绿原酸在0.118-1.18μg范围内线性关系良好（r=1.000 0）,平均加样回收率为97.9%,RSD为2.6%（n=5）;黄芩苷在0.069 6-0.556 8μg范围内线性关系良好（r=0.999 8）,平均加样回收率为98.2%,RSD为1.4%（n=5）。结论该法操作简单、准确、重现性好,可用于复方芩兰口服液的质量控制。
Objective To determine the contents of chlorogenic acid and Baicalin in fufangqinlan oral solution, Methods HPLC was performed by using C -18 column, The chlorogenic acid were separated with methanol -0, 4% phosphoric acid solution（ 24： 76 ） as a mobile phase and detected at 327 nm. The baicalin were separated with methanol - water - phosphoric acid solution（47： 53： 0.2） as a mobile phase and detected at 274 nm . Results The chlorogenic acid had good linear relationship（ r = 1. 000 0） within the range of 0.118 - 1.18 μg and the average recovery was 98.5 % with RSD of 1, 8 %, The baicalin had good linear relationship（ r = 0. 999 8 ） within the range of 0. 069 6 -0. 556 8μg and the average recovery was 98.2% with RSD of 1.4% . Conclusion The method is simple, accurate and reprodueible. It can be used for guality control of Fufangqinlan oral solution.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2008年第4期939-941,共3页
Lishizhen Medicine and Materia Medica Research
