摘要
以雏菊的根茎为试材,进行了根茎芽诱导和分化、分化芽的继代和生根、试管苗的移栽和移植的研究,建立起快速繁殖体系。结果表明:MS+6-BA 0.2 mg/L+GA30.5 mg/L+IAA0.2～0.5 mg/L是根茎诱导芽的理想培养基;MS+6-BA 1.0 mg/L+GA30.5 mg/L+NAA 0.1mg/L是根茎诱导芽分化培养和分化增殖培养的理想培养基;1/2MS+NAA 0.1 mg/L+IAA 0.4mg/L是分化芽生根培养的理想培养基;在温室中试管苗的移栽成活率为93.6%,移植到花坛上的试管苗保持了生长旺盛的优良性状。
In the experiment,the rhizome of Bellis perennis were used as material to do the research on adventitious buds induction and differentiation from rhizome,subculture and rooting of adventitious buds,transplanting of tube seedling,finally establish the rapid propagation system of Bellis perennis.The results showed that the optimum medium for adventitious buds induction and differentiation was MS＋6-BA 0.2 mg/L＋GA3 0.5 mg/L＋IAA 0.2~0.5 mg/L and MS＋6-BA 1.0 mg/L＋GA3 0.5 mg/L＋NAA 0.1 mg/L respectively.1/2MS＋NAA 0.1 mg/L＋IAA 0.4 mg/L was the optimum medium for rooting.When transplanting the tube seedlings into the greenhouse,the survival rate was 93.6%.The tube seedlings keep growing vigorously after transplanted to parterre.
出处
《北方园艺》
CAS
北大核心
2011年第12期110-112,共3页
Northern Horticulture
基金
辽宁省高等教育教学改革资助项目(20090304)
辽宁师范大学教学改革资助项目(LSJG:20090108)
关键词
雏菊
组织培养
快速繁殖
Bellis perennis
culture of tissue
rapid propagation
