摘要
目的评价免疫固定电泳、尿液轻链定量及血清游离轻链检测在多发性骨髓瘤(MM)患者轻链检测中的价值。方法用速率散射比浊法进行重链、轻链及游离轻链定量,用蛋白电泳及免疫固定电泳法对M成份做定性分析,对多发性骨髓瘤患者轻链型21例,完整型29例(其中IgGI、gA型19例;IgD型10例)标本分别做免疫固定电泳,尿轻链定量及游离轻链检测,然后比较三种方法的检出率和灵敏度。结果对于轻链型骨髓瘤患者,免疫固定电泳、尿轻链定量及血清游离轻链检测三种方法的轻链检测阳性率分别为90.5%(19/21);80.9%(17/21);100%(21/21)。对于完整型骨髓瘤患者三种方法的游离轻链检测(免疫固定电泳法检测与重链结合的单克隆轻链的阳性率100%)阳性率分别为20.7%(6/29);41.4%(12/29);82.7%(24/29)。结论血清游离轻链检测较另外两种方法检测游离轻链更为灵敏,其与免疫固定电泳法结合检测对多发性骨髓瘤患者的明确诊断、疗效观察及改善预后起重要作用。
Objective Evaluation of the tests of Immunofixation Electrophoresis（IFE）,urine light chain quantitive analysis and serum free light chain immunoassay for measurement of free light chain in multiple myeloma patients.Methods Turbidimetric method was used to quantify heavy chains,and total light chains（free and combined light chains）,protein electrophoresis（PE） and IFE were used to identify and type the M proteins in 21 light chain multiple myeloma（LCMM）and 29 intact immunoglobulin multiple myeloma（IIMM）patients;.Detection rate and accuracy of the three kinds of tests were compared.Results In LCMM patients,the detection rate for immunofixation Electrophoresis,urine light chain quantitive analysis and serum free light chain immunoassay were 90.5 %（19/21）;80.9 %（17/21）;100%（21/21）and were20.7 %（6/29）;41.4 %（12/29）;82.7 %（24/29）in IIMM patients,and all the combining monoclonal light chains were detected by IFE.Conclusion Serum free light chain immunoassay was more sensitive than the other two,if combining with IFE,they will help to diagnose,monitor and improve prognosis of multiple myeloma patients.
出处
《中国实验诊断学》
北大核心
2011年第9期1525-1527,共3页
Chinese Journal of Laboratory Diagnosis
关键词
多发性骨髓瘤
游离轻链
免疫固定电泳
multiple myeloma
free light chain
immunofixation electrophoresis
