摘要
选用长为316 bp的双链DNA片段研究其柔韧性和成环能力,并用两种核酸内切酶探测微环DNA结构的完整性.结果表明,在T_4连接酶的催化作用下,与Widom的报道一致,短双链DNA确实能够自发弯曲成环.用两种核酸酶——BAL31和S1作用之后,DNA环发生不同程度的消失,说明环状分子上存在非常规的缺陷结构.这种缺陷结构与短双链DNA的弯折刚性大大降低之间可能存在一定关系.
A 316 bp long fragment was used to form the mini-loop. Then the loop was treated by tow en donucleases to detect the unormal disruptions of the DNA helical structure. The results show that, a gree with Widom, short DNA fragments bend spontaneously into circles with the catalysis of T4 DNA ligase. The reason for the enhancement of flexibility may be that, local disruptions, which reduce the bending rigidity of DNA, occur during DNA cyclizing.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2011年第4期924-928,共5页
Journal of Sichuan University(Natural Science Edition)
基金
贵州大学研究生创新基金(校研农2007013)
贵州大学引进人才基金(贵大人基合字(2009)001)
省委组织部高层次人才特助基金(2006008)
贵州省社发攻关项目黔科合OZ字[2009]2
关键词
DNA拓扑结构
柔韧性
环化
局部缺陷
DNA, topological structure, flexibility, cyclization, local disruption
