摘要
目的探讨脊髓星形胶质细胞和小胶质细胞以及前炎性细胞因子白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)和肿瘤坏死因子α(TNFα)在CP/CPPS发生发展中的作用及机制。方法 40只健康雄性Sprague-Dawley(SD)大鼠,随机分为正常不加任何处理的对照组(n=20)、CP/CPPS模型组(n=20),完全福氏佐剂和3%角叉菜胶前列腺内注射造成CP/CPPS模型。通过检测机械性痛阈(PWT)来评价动物痛行为学改变;采用免疫组织化学、逆转录多聚酶链反应(RT-PCR)等方法,检测脊髓星形胶质细胞GFAP和小胶质细胞OX-42的表达以及脊髓前炎性细胞因子IL-1β、IL-6及TNFαmRNA表达的变化,评价脊髓小胶质细胞和星形胶质细胞活化情况及脊髓前炎性细胞因子表达与疼痛行为改变的关系。结果与对照组比较,CP/CPPS模型组大鼠建模后第11天出现PWT明显降低(P<0.05),并呈进行性下降趋势;随着时间进程,免疫组化结果显示模型组动物脊髓小胶质细胞和星形胶质细胞依次活化,小胶质细胞活化开始于建模后第7天,星形胶质细胞活化开始于建模后第14天;模型组动物脊髓IL-1β、IL-6和TNFαmRNA表达较对照组明显增加(P<0.05)。结论在CP/CPPS大鼠中脊髓胶质细胞活化及前炎性细胞因子IL-1β、IL-6和TNFα的表达增加,可能在神经病理性疼痛的产生和维持中起着重要的作用。
Objective To investigate the roles and mechanism of spinal glia and proinflammatory cytokinesIL-1β,IL-6 and TNF-α in the rat model of chronic prostatitis/chronic pelvic pain syndromes Methods SD rats were randomly divided into two groups（model group and control group）.The models of CP/CPPS were induced by injection of complete Freund＇S adjuvant and 3% carrageenan in prostate.The mechanical hyperalgesia（PWT） of the rats was measured,and the expression levels of IL-1β,IL-6 and TNF-α in the spinal cord of neuropathic pain models and controls were measured with histochemical staining and reverse transcription polymerase chain reaction（RT-PCR）.Results The progressive mechanical hyperalgesia happened after 11 days that CP/CPPS was built.Histochemical staining showed that the GFAP and OX-42 optical density ratios of the spinal dorsal horn were increased with time course after vincristine injection.The levels of IL-1β,IL-6 and TNF-α were up-regulated in the spinal cord with time course（P0.05）.Conclusion In the model of CP/CPPS,spinal microglia and astrocyte activations which promoted the release of IL-1β,IL-6 and TNF-α contribute to the maintenance of neuropathic pain.
出处
《中国实用医药》
2011年第23期1-3,共3页
China Practical Medicine
基金
福建省科技厅自然科学基金项目(项目编号:2008J0091
080916)
