期刊文献+

一株鸭新城疫强毒的分离鉴定和分子特性分析 被引量:5

Identification and Molecular Characteristics of One Strain of Newcastle Disease Virus Isolated from Duck
下载PDF
导出
摘要 从山东地区发病鸭中分离到一株新城疫病毒(SDLP09),经测定,其鸡胚半数致死量(ELD50)、鸡胚平均死亡时间(MDT)、1日龄雏鸡脑内接种致病指数(ICPI)、6周龄鸡静脉接种致病指数(IVPI)分别为107.85/0.1mL、48.5h、1.80、2.36,表明该新城疫病毒为强毒。通过分析其融合蛋白(F)发现,F蛋白多肽裂解位点为112RRQKRF117,符合NDV强毒株裂解位点氨基酸序列。F基因分型及氨基酸同源性比较发现,SDLP09株属于基因Ⅶ型,与野鸭源强毒NDV同源性更高,提示着该毒株可能来源于野鸭。 One strain of Newcastle disease virus (NDV) isolated from duck in Shandong was proved virulent in terms of median embryo lethal dose (ELD50) of 10^7.85/0.1 mL,mean death time (MDT) of 48.5 h,intracerebral pathogenicity index (ICPI) of 1.80,and intravenous pathogenicity index (IVPI) of 2.36. The cleavage site of F protein of the virus was 112RRQKRF117,and was accorded with highly pathogenic strain. By analyzing genotype and homology of F gene of SDLP09 and other NDV strains, it showed that SDLP09 belonged to genotype Ⅶ,and had higher homology with virulent NDV from wild duck.
出处 《中国家禽》 北大核心 2011年第16期13-15,共3页 China Poultry
关键词 新城疫病毒 F蛋白 分子特性 duck Newcastle disease virus F protein molecular characteristics
  • 相关文献

参考文献7

二级参考文献29

  • 1王莉林,霍龙飞,曹殿军,等.新城疫病毒融合蛋白基因(F)的克隆与鉴定[A].1995年全国首届分子生物学研讨会论文汇编[C].扬州:1995.28~30. 被引量:1
  • 2Collins M S,Strong I,Alexander D J, et al.P-athogenicity and p hylogeneticevaluation of the variant Newcastle disease viruses termed "pigeon PMV-1 viruse s" based on the nucleotide sequence of the fusion protein gene[J]. Arch Virol,1996,141:635~747. 被引量:1
  • 3Werner O, Romer-Oberdorfer A, Kollner B,et al. Characterization of avian paramyxovirus type 1 strains isolated in Germany during 1992 to 1996[J].Avian Pathology,1999,28:79~88. 被引量:1
  • 4Mcginnes,Wilde A,Morrison,et al.Nucleotide sequence of the gene encoding theNewcastle disease virus haemagglutinitein and comparisons of paramyxovirus haemagglutinin-neuraminidase protein sequence[J].Virus research ,1987,7:187~202 . 被引量:1
  • 5Alexander D J,Manvell,Lowings,et al.Antigenic diversity and simi larities inavian paramyxovirus type 1( Newcastle disease virus) isolate using monoclonal antibodies[J].Avian Pathology,1998,27:399~418. 被引量:1
  • 6Ballagi-Pordany,Wehmann,Herczeg, et al. Identification and group ing of Newc astledisease virus strains by restriction site analysis of a region from the F gene[J]. ArchVirol,1996,141:243~261. 被引量:1
  • 7Yang Cheng-Yao, Shieh Happy K, Lin Yeou-liang,et al. Newcastle disease viru sisolated from recent outbreaks in taiwan phylogenetically related to viruses ( genotypeⅦ) from recent outbreaks in Westrern Europe[J]. Avian Disease,1999,4 3:125~130. 被引量:1
  • 8Ballagi-Pordany, Wehmann, Herczeg J, et al. N-ewcastle diseas e outbreaks inrecent years in Western Europe were caused by an old (Ⅵ) and a novel genotype ( Ⅶ)[J].Arch Virol,1998,43:49~64. 被引量:1
  • 9Jarecki-Black,Bennett,Palmier S. A novel oligonucl-eotide pro be for the detectionof DNC[J]. Avian Disease ,1992,36:134~138 . 被引量:1
  • 10贺东生,宋长绪,刘福安,等.用核酸探针检测新城疫病毒的研究[A].中国畜牧兽医学会禽病学分会第八次学术讨论会论文集(续集)[C].青岛:1996.44~46. 被引量:1

共引文献114

同被引文献48

  • 1程龙飞,柯美峰,郑腾,黄瑜,李文杨,施少华.猪源新城疫病毒的分离鉴定[J].中国兽医科技,2004,34(12):66-68. 被引量:12
  • 2黄兵,马秀丽,王莉莉,李玉峰,刘玉山,陈溥言.H_9与H_5亚型禽流感病毒血凝素基因的快速鉴别[J].中国病毒学,2005,20(5):498-502. 被引量:4
  • 3李建军,丁巧玲,袁生,袁圣丹,万碧燕,朱晓玲.鸭副黏病毒的分离与鉴定[J].中国家禽,2006,28(24):135-136. 被引量:2
  • 4辛朝安,任涛,罗开健,张洁,杨佩琼.疑似鹅副粘病毒感染诊断初报[J].养禽与禽病防治,1997(1):5-5. 被引量:87
  • 5Roemer-OberdoerferA, Werner O, Veits J, et al. Contribution of the length of the HN protein and the sequence of the F protein cleavage site to Newcastle disease virus pathogenicity. J Gen Virol, 2003, 84 (11): 3121-3129. 被引量:1
  • 6Alexander D J.Newcastle disease and other avian Paramyxoviridae infeetions[M]//Calnek B W.Diseases of Poultry.10th ed.Ames, IA. Iowa State University Press, 1997: 541-569. 被引量:1
  • 7OIE.哺乳动物、禽类和蜜蜂A类和B类疾病诊断试验和疫苗标准手册[M].农业部畜牧兽医局,译.北京:中国农业科技出版社出版,1996:140-146. 被引量:3
  • 8Takehara K, Shinomiya T, Kobayashi H, et al. Characterization of Newcastle disease virus isolated from field cases in Japan[J].Avian Diseases, 1987, 31 (1): 125-129. 被引量:1
  • 9Huovilainen A, Ek-Kommonen C, Manvell R, et al. Phylogenetic analysis of avian paramyxovirus 1 strains isolated in Finland[J].ArchivesofVirology, 2001, 146 (9): 1775-1785. 被引量:1
  • 10Gould A R, Kattenbeldt J A, Selleck P, et al.Virulent Newcastle in Australia: molecular epidemiological analysis of viruses isolated prior to and during the outbreak of 1998-2000[J]. Virus Research, 2001, 77 (1): 51-60. 被引量:1

引证文献5

二级引证文献11

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部