摘要
目的:探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)信号途径在多囊卵巢综合征(PCOS)大鼠卵巢颗粒细胞诱导凋亡的最终效应阶段的作用。方法:采用硫酸普拉睾酮钠诱导大鼠PCOS模型,采用Western blot及RT-PCR分析半胱氨酸天门冬氨酸蛋白酶3(Caspase3)在PCOS大鼠卵巢颗粒细胞的表达情况。结果:①Western blot分析显示,PCOS组大鼠卵巢颗粒细胞活性Caspase3蛋白的表达较对照组明显增强(P<0.01)。②RT-PCR分析显示,PCOS组大鼠卵巢颗粒细胞Caspase3mRNA的表达较对照组明显增强(P<0.01)。结论:TRAIL信号途径在PCOS大鼠颗粒细胞诱导的凋亡可能最终经过了Caspase级联反应,引起Caspase3的激活导致细胞凋亡。
Objective：To explore the role of tumor necrosis factor-related apoptosis-inducing ligand（TRAIL） signal pathway in the final effective stage of apoptosis of granulosa cells of rats with polycystic ovary syndrome（PCOS）. Methods：Sodium prasterone sulfate was used to induce rat models of PCOS,Western blot and RT-PCR were used to analyze the expression of Caspase3 in granulosa cells of rats with PCOS. Results：Western blot analysis showed that the expression level of actived Caspase3 protein in PCOS group was significantly higher than that in control group（P0.01）.RT-PCR analysis showed that the expression level of Caspase3 mRNA in PCOS group was significantly higher than that in control group（P0.01）. Conclusion：TRAIL signal pathway may pass Caspase cascade finally during the apoptosis of granulosa cells of rats with PCOS,induce cell apoptosis by activating Caspase3.
出处
《中国妇幼保健》
CAS
北大核心
2011年第24期3798-3800,共3页
Maternal and Child Health Care of China
