摘要
目的:探讨人工冬虫夏草(虫草菌液)联合糖皮质激素对博莱霉素诱导的大鼠肺纤维化的干预作用。方法:50只SD大鼠,随机分为5组:正常对照组、模型组、虫草菌液组、强的松组、虫草菌液联合强的松组(联合组)。采用气管内注射博莱霉素建立模型,次日起分别给药灌胃治疗,正常组和模型组给予生理盐水。第28天处死动物,肺组织标本行HE染色及MASSON染色进行病理形态学观察、Ashcroft纤维化评分,碱水解法检测肺组织HYP含量,免疫组化检测肺组织CTGF蛋白表达,酶联免疫法检测肺泡灌洗液上清TGF-β1含量。结果:与模型组相比,虫草菌液、强的松组能够降低肺组织纤维化评分,减少肺组织胶原沉积、HYP含量,下调肺组织CTGF蛋白表达,降低BLAF中TGF-β1的含量;联合组分别与虫草菌液组、强的松组比较,能够进一步减少肺纤维化评分、胶原面积、HYP含量,进一步下调CTGF蛋白、TGF-β1蛋白表达。结论:虫草菌液、强的松对博莱霉素诱导的大鼠肺纤维化有治疗作用,且虫草菌液联合强的松能产生协同治疗效果。
Objective： To study the treatment effects of cultured Cordyceps sinensis combined with glucocorticosteroid on experimental pulmonary fibrosis in rats induced by bleomycin.Method： Fifty rats were randomly divided into five groups,including control group,model group,cultured C.sinensis groups,prednisone group,cultured C.sinensis combined with prednisone group.On experimental day 0,the rats were respectively intratracheally instilled with bleomycin,and rats in the control group and model group with the same volume of normal saline.One day after the injection,cultured C.sinensis and glucocorticosteroid was respectively given to rats daily by gastric gavage,while the same volume of normal saline was given to those in the control group and model group.On 28th d,bronchoalveolar lavage fluid（BALF） and lung tissue were collected.Histological changes of the lungs were evaluated by HE stain,Masson′s trichrome stain.Collagen content of the lung tissue was assessed by hydroxyprolin concentration.Lung expression of CTGF protein was assessed by immunohistochemistry.The level of TGF-β1 protein was measured by ELISA.Result： Compared to model group,pulmonary fibrosis were alleviated in cultured C.sinensis and prednisone group,and CTGF expression,Hydroxyproline concentrations and protein TGF-β1 were decreased.The combination effect of C.sinensis and prednisone group is augmented compared with using C.sinensis or prednisone group alone.Conclusion： The cultured C.sinensis and prednisone alleviates pulmonary fibrosis,and the combination use of both drugs has synergia effects in anti-fibrous degeneration.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2011年第16期2265-2270,共6页
China Journal of Chinese Materia Medica
关键词
人工冬虫夏草
博莱霉素
肺纤维化
强的松
协同作用
cultured Cordyceps sinensis
bleomycin
pulmonary fibrosis
prednisone
synergia effect
