摘要
目的探讨姜黄素抑制人宫颈癌HeLa细胞增殖的内在机制。方法用不同浓度的姜黄素作用HeLa细胞,在作用的不同时间内用MTT法测定姜黄素对HeLa细胞抑制增殖的效应;RT-PCR法检测P53 mRNA的表达;Western blot法检测乙酰化组蛋白H3、乙酰化P53以及P53蛋白的表达。结果姜黄素对HeLa细胞增殖有明显抑制作用,不仅有剂量依赖性,还存在明显的时间依赖性;而且姜黄素能明显上调HeLa细胞内组蛋白H3的乙酰化水平,促进P53的乙酰化、P53基因mRNA及相应蛋白的表达。结论姜黄素通过上调组蛋白H3乙酰化水平,促进肿瘤抑制因子P53的活化与表达来抑制宫颈癌HeLa细胞的增殖。姜黄素具有去乙酰化酶抑制剂作用,有望开发用于治疗宫颈癌。
Objective To investigate the cytotoxicity and proapoptotic activity of Curcumin on cervical cancer cell line Hela and its mocular mechanisms.Methods HeLa cells were treated with different concentrations of Curcumin for different time,MTT assay was performed to examin the growth inhibition effects of Curcumin on HeLa cells.The expression of P53 was assayed by reverse transcriptase polymerase chain reaction（RT-PCR）.The expressions of acetylated histone H3,P53 and acetylated P53 protein were determined by Western blot.Results Curcumin could inhibit the proliferation of HeLa cells in a time-and-dose-dependent manner.The expression of P53 mRNA was different with the concentration and time of Curcumin,which was the strongest in 25 μmol/L at 48 h.The levels of histone H3 acetylation,P53 expression and P53 acetylation were increased significantly when treated with different concentrations of Curcumin for different time.Conclusion Curcumin functions as a deacetylase inhibitor,which could increase the level of acetylated histone H3,enhance the expression and activity of tumor suppressor P53 and inhibit the proliferation of cervical cancer cell line HeLa.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2011年第8期899-902,共4页
Cancer Research on Prevention and Treatment
