摘要
目的:评价针刺督脉穴对血管性痴呆(Vascular dementia,VD)大鼠模型的学习记忆改善情况及可能机制,为针刺治疗血管性痴呆提供理论依据。方法:采用两侧颈总动脉血管阻断加硝普钠降压法复制VD大鼠模型;对VD大鼠模型进行西药(喜得镇)、针刺和电针干预;采用morris水迷宫法观察学习记忆情况;应用免疫组化法测定海马CA1区Bcl-2、Bax蛋白表达及原位末端标记法检测细胞凋亡。结果:(1)morris水迷宫学习记忆能力检测,经过6次训练,与对照组比较,模型组跳台时间延长;与模型组比较,各治疗组跳台时间明显缩短,差异均有统计学意义(P<0.05)。(2)凋亡相关蛋白检测结果显示:与对照组比较,模型组海马组织bcl-2、bax及细胞凋亡明显增高,差异均有统计学意义(P<0.01);与模型组比较,各治疗组海马组织bcl-2水平增高,bax及细胞凋亡水平明显降低,差异均有统计学意义(P<0.01)。结论:(1)各治疗组均可改善VD模型大鼠的认知能力,提高学习记忆成绩。(2)各治疗组均可抑制VD大鼠海马组织细胞凋亡。
Objective：Evaluating the effect of acusector on Du Channel on improving memory and its mechanism, in order to provide theory basis in treatment Vascular dementia. Methods：Estabolishing Vascular dementia model by interdicting carotids in two sides combined with sodium nitroprusside. Treatment Vascular dementia with hydergine, acupuncture and acusector. Observed leaning memory by the method of water maze. Determined bcl - 2 and Bax expression and apoptosis by immunohistochemistry and TUNEL respectively. Results ： （ 1 ） The time of jump pahform was longer in the group of model compared with control, it was shorter in each of treatment groups compared with model group, the difference of them was significant（ P 〈 0. 05 ）, （2） The acetylcholinesterase activity in model group was significantly increased when compared with control （P 〈 0.01 ）. There was a de- creased trend in acetylcholinesterase activity in each of treatment groups （P 〉 0. 05 ）. （4） The expression of bcl -2 and bax and the apoptosis were significantly increased in model group compared with control （P 〈 0. 01 ）. The expression of bcl -2 was significantly increased,but bax and apoptosis were significantly decreased （P 〈0. 01 ）. Conclusion： （ 1 ） Each of treatment grops could improved the ability of cognition of rat vascular dementia, and increased achievement of learning and memory. （2） Each of treatment groups could inhibit apoptosis in hippocampi tissue of rat vascular dementia.
出处
《辽宁中医杂志》
CAS
北大核心
2011年第8期1667-1670,共4页
Liaoning Journal of Traditional Chinese Medicine
基金
辽宁省重点专科建设项目
