摘要
目的:探讨川芎嗪预处理对大鼠心肌缺血再灌注损伤的保护作用。方法:将30只Wistar大鼠随机分为3组:假手术组、缺血再灌注损伤组、川芎嗪预处理组。建立大鼠心肌缺血再灌注损伤模型,测定SOD、GSH-Px活性,应用原位杂交技术和免疫组化S-P法检测HSP70蛋白的表达。结果:与缺血再灌注损伤组比较,川芎嗪预处理组SOD(P<0.01)、GSH-Px(P<0.05)活力显著升高,HSP70mRNA(P<0.01)及其蛋白(P<0.05)的表达显著增加。结论:SOD、GSH-Px和HSP70是心肌中重要的内源性保护物质,川芎嗪可增加SOD、GSH-Px活性,并诱导HSP70mRNA及其蛋白的表达而发挥心肌保护作用。
Objective： To probe into the protective effect of ehuanxiongzine pretreatrnent on rats＇ myocardial ischemia reperfusion injury. Methocks： 30 Wistar mrs were randomly divided into 3 groups： sham operation group, the group of isehemia reperfusion injury and the group of ehuanxiongzine pretreatment. The model rats with myocardial tsehemia reperfusion injury was built and ,SOD and GSH- Px activity were detected. In situ hybridization and Streptavidin-peroxidase（S- P） immunohistoehemieal rnethod were used to detect the expression of heat shock protein 70. Results： Compared with the group of ischemia reperfusion injury, the group of ehuanxionffzine pretreatment had an evident increase of vatc.lity： .SOD （P〈 0.01 ） and GSH- Px （P〈 0.05）, and a remarkably increased experssion of HSP70 mRNA（P 〈 0.0 1 ） and protine（P 〈 0.05）. Conclusion： SOD, GSH - Px and HSPT0 were the important endogenous protective sulxstanees in the myocardium. Chuanxiongzine can enhance the activity of SOD and GSH - Px and induee the HSP70mRNA and the expression of its protein, which was the protective effect of mycxzardium.
出处
《河南中医》
2008年第4期26-28,共3页
Henan Traditional Chinese Medicine
基金
河南中医学院苗圃项目(编号:2006-6)
关键词
川芎嗪
缺血再灌注损伤
HSPT0
原位杂交技术
免疫组化
大鼠
ehuanxiongzine
ischernia reperfusion injury
I-LSP70
In situ hybridization
Streptavidin-pemxidase immunohistochermical method
rats
