摘要
目的:观察电针对血管性痴呆大鼠学习记忆能力和突触超微结构的影响。方法:实验于2005-08/10在广州中医药大学动物实验中心完成。选择SPF级成年健康SD大鼠50只,随机抽取8只大鼠为假手术组,其余42只采用四血管阻断法制备缺血模型。将造模成功的大鼠按随机数字表法分为电针组,尼莫通组和模型组。假手术组和模型组同等条件下饲养,未予任何治疗。电针组:用28号1寸毫针,于模型大鼠头部“百会”穴(顶骨正中)斜刺0.5寸,背部膈俞穴(第7胸椎下两旁肋间)、脾俞穴(第12胸椎下两旁肋间)和肾俞穴(第2腰椎下两旁),各直刺0.5寸,连接电针仪,施以连续波,频率150Hz,强度以大鼠安静耐受为度(约1mA),1次/d,留针20min,连续治疗15d。尼莫通组:大鼠给予尼莫通12mg/kg,按20mL/kg灌胃,1次/d,连续15d。治疗15d后采用Morris水迷宫测定大鼠学习记忆能力,包括定位航行试验和空间探索试验;应用透射电镜和图像分析系统测量GrayⅠ型突触的面数密度、面积密度、体积密度变化。结果:纳入动物50只,32只进入结果分析,制备动物模型42只中术后7d内死亡15只,存活27只,其中2只因术后出现偏瘫不能进行水迷宫测试而弃之不用。25只动物造模成功,其中电针组9只,尼莫通组9只和模型组7只。假手术组死亡1只,存活7只。①模型组大鼠表现明显的学习记忆障碍,在水迷宫定位航行试验中,其逃避潜伏期明显长于假手术组、电针组、尼莫通组[分别为(16.89±9.13),(5.20±0.81),(5.49±0.90),(6.73±0.97)s,P<0.01],电针组大鼠逃避潜伏期明显缩短(P<0.05);在水迷宫空间探索试验中,模型组在原平台象限跨越平台次数与其余3个象限无显著差异,电针组相同时间内跨越原平台次数明显多于其余3个象限[原平台、右侧平台、左侧平台、对侧平台分别为(5.67±1.50),(1.33±1.00),(0.89±0.78),(1.11±0.78)次,P<0.01]。②假手术组、电针组和尼莫通组大
AIM： To observe the effect of electro-acupuncture （EA） on the learning and memory abilities and the synapse ultrastructure in CA3 sector of the hippocampus on vascular dementia （VD） rats. METHODS： The experiment was conducted in the Experimental Animals Center of Guangzhou University of Traditional Chinese Medicine from August to October in 2005. Fifty adult healthy SD rats of SPF grade were adopted in this study, and 8 of them were randomly selected as sham group while other 42 rats were used to prepared global ischemia reperfusion models by 4 vascular occlusions. Then the successful model rats were randomized into EA group, nimotop group and model group. The rats of sham group and model group were fed normally without any treatment. EA group： A No.28 one-inch needle was applied to prick inclined into right middle of parietal bone Baihui （DU 20） for 0.5 inches, and directly onto Geshu （BL 17）, Pishu （BL 20） and Shenzhu （BL 23） for 0.5 inches with EA apparatus in continuous wave of 150 Hz frequency, once a day for successive 15 days. The needle was retained for 20 minutes and the intensity was suitable for the rats＇ quiet tolerance （about 1 mA）. Nimotop group： The rats were injected intragastrically with 20 mL/kg nimotop （12 mg/kg）, once a day for successive 15 days. Morris water maze were used to test the learning and memory abilities, including place navigation test and spatial probe test. The changes of numerical density, surface density and volume density of Gray type Ⅰ synapses in CA3 sector of the hippocampus were measured with transmission electron microscope and picture analysis system.
RESULTS： Totally 32 of 50 rats were involved in the result analysis. Among 42 rats used for modeling, 15 died within postoperative 7 days and 2 were deleted due to hemiplegia appearance while 25 ones were successful models, containing 9 in EA group, 9 in nimotop group and 7 in model group. In addition, there were seven survivals and one death in sham group. ①The animals of m
出处
《中国临床康复》
CSCD
北大核心
2006年第39期42-45,F0003,共5页
Chinese Journal of Clinical Rehabilitation
